論文 - 詳細
| RRC ID | 30356 |
|---|---|
| 著者 | Kobayashi T, Takahara M, Miyagishima SY, Kuroiwa H, Sasaki N, Ohta N, Matsuzaki M, Kuroiwa T. |
| タイトル | Detection and localization of a chloroplast-encoded HU-like protein that organizes chloroplast nucleoids. |
| ジャーナル | Plant Cell |
| Abstract |
Chloroplast DNA (cpDNA) is packed into discrete structures called chloroplast nucleoids (cp-nucleoids). The structure of cpDNA is thought to be important for its maintenance and regulation. In bacteria and mitochondria, histone-like proteins (such as HU and Abf2, respectively) are abundant and play important roles in DNA organization. However, a primary structural protein has yet to be found in cp-nucleoids. Here, we identified an abundant DNA binding protein from isolated cp-nucleoids of the primitive red alga Cyanidioschyzon merolae. The purified protein had sequence homology with the bacterial histone-like protein HU, and it complemented HU-lacking Escherichia coli mutants. The protein, called HC (histone-like protein of chloroplast), was encoded by a single gene (CmhupA) in the C. merolae chloroplast genome. Using immunofluorescence and immunoelectron microscopy, we demonstrated that HC was distributed uniformly throughout the entire cp-nucleoid. The protein was expressed constitutively throughout the cell and the chloroplast division cycle, and it was able to condense DNA. These results indicate that HC, a bacteria-derived histone-like protein, primarily organizes cpDNA into the nucleoid. |
| 巻・号 | 14(7) |
| ページ | 1579-89 |
| 公開日 | 2002-7-1 |
| DOI | 10.1105/tpc.002717 |
| PMID | 12119376 |
| PMC | PMC150708 |
| MeSH | Algal Proteins / genetics Algal Proteins / metabolism* Amino Acid Sequence Bacterial Proteins / genetics Chloroplasts / metabolism* DNA, Chloroplast / genetics* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Escherichia coli / genetics Genetic Complementation Test Immunoblotting Molecular Sequence Data Mutation Rhodophyta / genetics* Rhodophyta / metabolism Saccharomyces cerevisiae Proteins* Sequence Homology, Amino Acid Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 9.618 |
| 引用数 | 54 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 藻類 | NIES-3377 |