論文 - 詳細
| RRC ID | 30374 |
|---|---|
| 著者 | Kobayashi Y, Kanesaki Y, Tanaka A, Kuroiwa H, Kuroiwa T, Tanaka K. |
| タイトル | Tetrapyrrole signal as a cell-cycle coordinator from organelle to nuclear DNA replication in plant cells. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Eukaryotic cells arose from an ancient endosymbiotic association of prokaryotes, with plant cells harboring 3 genomes as the remnants of such evolution. In plant cells, plastid and mitochondrial DNA replication [organelle DNA replication (ODR)] occurs in advance of the subsequent cell cycles composed of nuclear DNA replication (NDR) and cell division. However, the mechanism by which replication of these genomes with different origins is coordinated is largely unknown. Here, we show that NDR is regulated by a tetrapyrrole signal in plant cells, which has been suggested as an organelle-to-nucleus retrograde signal. In synchronized cultures of the primitive red alga Cyanidioschyzon merolae, specific inhibition of A-type cyclin-dependent kinase (CDKA) prevented NDR but not ODR after onset of the cell cycle. In contrast, inhibition of ODR by nalidixic acid also resulted in inhibition of NDR, indicating a strict dependence of NDR on ODR. The requirement of ODR for NDR was bypassed by addition of the tetrapyrrole intermediates protoporphyrin IX (ProtoIX) or Mg-ProtoIX, both of which activated CDKA without inducing ODR. This scheme was also observed in cultured tobacco cells (BY-2), where inhibition of ODR by nalidixic acid prevented CDKA activation and NDR, and these inhibitions were circumvented by Mg-ProtoIX without inducing ODR. We thus show that tetrapyrrole-mediated organelle-nucleus replicational coupling is an evolutionary conserved process among plant cells. |
| 巻・号 | 106(3) |
| ページ | 803-7 |
| 公開日 | 2009-1-20 |
| DOI | 10.1073/pnas.0804270105 |
| PII | 0804270105 |
| PMID | 19141634 |
| PMC | PMC2625283 |
| MeSH | Cell Cycle* Cell Nucleus / metabolism* Cyclin-Dependent Kinases / physiology DNA Replication* Organelles / metabolism* Plants / genetics* Rhodophyta / genetics* Tetrapyrroles / physiology* |
| IF | 9.412 |
| 引用数 | 82 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-3377 |