論文 - 詳細
| RRC ID | 30377 |
|---|---|
| 著者 | Nishida K, Yagisawa F, Kuroiwa H, Nagata T, Kuroiwa T. |
| タイトル | Cell cycle-regulated, microtubule-independent organelle division in Cyanidioschyzon merolae. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Mitochondrial and chloroplast division controls the number and morphology of organelles, but how cells regulate organelle division remains to be clarified. Here, we show that each step of mitochondrial and chloroplast division is closely associated with the cell cycle in Cyanidioschyzon merolae. Electron microscopy revealed direct associations between the spindle pole bodies and mitochondria, suggesting that mitochondrial distribution is physically coupled with mitosis. Interconnected organelles were fractionated under microtubule-stabilizing condition. Immunoblotting analysis revealed that the protein levels required for organelle division increased before microtubule changes upon cell division, indicating that regulation of protein expression for organelle division is distinct from that of cytokinesis. At the mitochondrial division site, dynamin stuck to one of the divided mitochondria and was spatially associated with the tip of a microtubule stretching from the other one. Inhibition of microtubule organization, proteasome activity or DNA synthesis, respectively, induced arrested cells with divided but shrunk mitochondria, with divided and segregated mitochondria, or with incomplete mitochondrial division restrained at the final severance, and repetitive chloroplast division. The results indicated that mitochondrial morphology and segregation but not division depend on microtubules and implied that the division processes of the two organelles are regulated at distinct checkpoints. |
| 巻・号 | 16(5) |
| ページ | 2493-502 |
| 公開日 | 2005-5-1 |
| DOI | 10.1091/mbc.e05-01-0068 |
| PII | E05-01-0068 |
| PMID | 15772156 |
| PMC | PMC1087252 |
| MeSH | Algal Proteins / metabolism Base Sequence Cell Cycle Cell Division Chloroplasts / ultrastructure DNA Replication / drug effects DNA, Algal / biosynthesis DNA, Algal / genetics Dynamin I / metabolism Microscopy, Electron Microtubules / drug effects Microtubules / ultrastructure Organelles / ultrastructure Proteasome Inhibitors Rhodophyta / cytology Rhodophyta / genetics Rhodophyta / metabolism Rhodophyta / ultrastructure* Spindle Apparatus / ultrastructure |
| IF | 3.791 |
| 引用数 | 43 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-3377 |