RRC ID 30384
Author Ohnuma M, Yokoyama T, Inouye T, Sekine Y, Tanaka K.
Title Polyethylene glycol (PEG)-mediated transient gene expression in a red alga, Cyanidioschyzon merolae 10D.
Journal Plant Cell Physiol.
Abstract DNA introduction into cells is an essential technique for molecular genetic analysis. Here, we show that DNA is easily introduced into cells of the unicellular red alga Cyanidioschyzon merolae by a polyethylene glycol (PEG)-mediated protocol. In this study, the beta-tubulin gene of C. merolae was cloned on a plasmid and a hemagglutinin (HA) tag then added at the C-terminus. This plasmid was then introduced into C. merolae cells by a PEG-mediated transformation protocol. At 24 h after PEG-mediated transformation, intracellular localization of the tagged protein was detected by anti-HA immunocytochemistry, indicating the utility of this transient expression system for molecular genetic analyses.
Volume 49(1)
Pages 117-20
Published 2008-1
DOI 10.1093/pcp/pcm157
PII pcm157
PMID 18003671
MeSH Amino Acid Sequence Base Sequence DNA / genetics DNA / metabolism Gene Expression* Molecular Sequence Data Plasmids / genetics Plasmids / metabolism Polyethylene Glycols* Rhodophyta / genetics* Transfection / methods*
IF 4.059
Times Cited 53
WOS Category PLANT SCIENCES CELL BIOLOGY
Resource
Algae NIES-3377