Reference - Detail
|Author||Ohnuma M, Yokoyama T, Inouye T, Sekine Y, Tanaka K.|
|Title||Polyethylene glycol (PEG)-mediated transient gene expression in a red alga, Cyanidioschyzon merolae 10D.|
|Journal||Plant Cell Physiol.|
DNA introduction into cells is an essential technique for molecular genetic analysis. Here, we show that DNA is easily introduced into cells of the unicellular red alga Cyanidioschyzon merolae by a polyethylene glycol (PEG)-mediated protocol. In this study, the beta-tubulin gene of C. merolae was cloned on a plasmid and a hemagglutinin (HA) tag then added at the C-terminus. This plasmid was then introduced into C. merolae cells by a PEG-mediated transformation protocol. At 24 h after PEG-mediated transformation, intracellular localization of the tagged protein was detected by anti-HA immunocytochemistry, indicating the utility of this transient expression system for molecular genetic analyses.
|MeSH||Amino Acid Sequence Base Sequence DNA / genetics DNA / metabolism Gene Expression* Molecular Sequence Data Plasmids / genetics Plasmids / metabolism Polyethylene Glycols* Rhodophyta / genetics* Transfection / methods*|
|WOS Category||PLANT SCIENCES CELL BIOLOGY|