Reference - Detail
RRC ID | 30384 |
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Author | Ohnuma M, Yokoyama T, Inouye T, Sekine Y, Tanaka K. |
Title | Polyethylene glycol (PEG)-mediated transient gene expression in a red alga, Cyanidioschyzon merolae 10D. |
Journal | Plant Cell Physiol |
Abstract |
DNA introduction into cells is an essential technique for molecular genetic analysis. Here, we show that DNA is easily introduced into cells of the unicellular red alga Cyanidioschyzon merolae by a polyethylene glycol (PEG)-mediated protocol. In this study, the beta-tubulin gene of C. merolae was cloned on a plasmid and a hemagglutinin (HA) tag then added at the C-terminus. This plasmid was then introduced into C. merolae cells by a PEG-mediated transformation protocol. At 24 h after PEG-mediated transformation, intracellular localization of the tagged protein was detected by anti-HA immunocytochemistry, indicating the utility of this transient expression system for molecular genetic analyses. |
Volume | 49(1) |
Pages | 117-20 |
Published | 2008-1-1 |
DOI | 10.1093/pcp/pcm157 |
PII | pcm157 |
PMID | 18003671 |
MeSH | Amino Acid Sequence Base Sequence DNA / genetics DNA / metabolism Gene Expression* Molecular Sequence Data Plasmids / genetics Plasmids / metabolism Polyethylene Glycols* Rhodophyta / genetics* Transfection / methods* |
IF | 4.062 |
Times Cited | 76 |
WOS Category | PLANT SCIENCES CELL BIOLOGY |
Resource | |
Algae | NIES-3377 |