論文 - 詳細
| RRC ID | 30384 |
|---|---|
| 著者 | Ohnuma M, Yokoyama T, Inouye T, Sekine Y, Tanaka K. |
| タイトル | Polyethylene glycol (PEG)-mediated transient gene expression in a red alga, Cyanidioschyzon merolae 10D. |
| ジャーナル | Plant Cell Physiol |
| Abstract |
DNA introduction into cells is an essential technique for molecular genetic analysis. Here, we show that DNA is easily introduced into cells of the unicellular red alga Cyanidioschyzon merolae by a polyethylene glycol (PEG)-mediated protocol. In this study, the beta-tubulin gene of C. merolae was cloned on a plasmid and a hemagglutinin (HA) tag then added at the C-terminus. This plasmid was then introduced into C. merolae cells by a PEG-mediated transformation protocol. At 24 h after PEG-mediated transformation, intracellular localization of the tagged protein was detected by anti-HA immunocytochemistry, indicating the utility of this transient expression system for molecular genetic analyses. |
| 巻・号 | 49(1) |
| ページ | 117-20 |
| 公開日 | 2008-1-1 |
| DOI | 10.1093/pcp/pcm157 |
| PII | pcm157 |
| PMID | 18003671 |
| MeSH | Amino Acid Sequence Base Sequence DNA / genetics DNA / metabolism Gene Expression* Molecular Sequence Data Plasmids / genetics Plasmids / metabolism Polyethylene Glycols* Rhodophyta / genetics* Transfection / methods* |
| IF | 4.062 |
| 引用数 | 76 |
| WOS 分野 | PLANT SCIENCES CELL BIOLOGY |
| リソース情報 | |
| 藻類 | NIES-3377 |