| RRC ID |
30408
|
| Author |
Moffat KG, Gould JH, Smith HK, O'Kane CJ.
|
| Title |
Inducible cell ablation in Drosophila by cold-sensitive ricin A chain.
|
| Journal |
Development
|
| Abstract |
We have developed a system for temperature-inducible killing of specific cells in the fruitfly Drosophila melanogaster. The system overcomes many of the limitations of existing cell ablation methods and is in principle applicable to any non-homeothermic eukaryote. Temperature-sensitive and cold-sensitive mutations in the ricin toxin A chain (RTA) of castor bean were generated in yeast. One cold-sensitive mutation, RAcs2, produced temperature-dependent ablation of eye cells in Drosophila when expressed under control of the eye-specific sev enhancer. At 29 degrees C, cell death was observed within 7 hours in the developing eye and no obvious toxic effects were observed elsewhere; at 18 degrees C, extremely low toxicity was observed. DNA sequencing of RAcs2 revealed a single amino acid substitution in the RTA active site cleft.
|
| Volume |
114(3)
|
| Pages |
681-7
|
| Published |
1992-3-1
|
| DOI |
10.1242/dev.114.3.681
|
| PMID |
1618135
|
| MeSH |
Animals
Cell Death / genetics*
Cold Temperature*
Drosophila melanogaster / genetics*
Eye / cytology
Eye / embryology
Eye / ultrastructure
Genes, Lethal / physiology*
Microscopy, Electron, Scanning
Ricin / genetics*
|
| IF |
5.611
|
| Times Cited |
64
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Drosophila |
DGRC#109670 |
