RRC ID 30410
Author Frölich A, Würgler FE.
Title New tester strains with improved bioactivation capacity for the Drosophila wing-spot test.
Journal Mutat. Res.
Abstract In Drosophila melanogaster new tester strains for the somatic mutation and recombination test (SMART) in the wing were constructed with the aim of increasing the metabolic capacity to activate promutagens. Some aspects of the genetic control of the xenobiotics metabolism in Drosophila are already known. In the DDT-resistant strain Oregon R(R) the RI gene at position 65.0 on chromosome 2 is responsible for the high constitutive expression of cytochrome P-450-dependent activities typical for this strain. Therefore, chromosomes 1 and 2 in the original mwh (multiple wing hairs) and flr3 (flare3) tester strains were substituted for chromosomes 1 and 2 from the Oregon R(R) strain. In assays with the model promutagen diethylnitrosamine an increased sensitivity of about 2.5-fold was found for this new set of 'HB' strains ('High Bioactivation (HB) cross').
Volume 216(3)
Pages 179-87
Published 1989-6
PII 0165-1161(89)90003-4
PMID 2499781
MeSH Animals Biotransformation Diethylnitrosamine / toxicity Dose-Response Relationship, Drug Drosophila melanogaster / drug effects Drosophila melanogaster / genetics* Mutagenicity Tests / methods* Wings, Animal
IF 2.011
Times Cited 64
WOS Category TOXICOLOGY GENETICS & HEREDITY
Resource
Drosophila DGRC#109612