| RRC ID |
30447
|
| 著者 |
Okada M, Akimaru H, Hou DX, Takahashi T, Ishii S.
|
| タイトル |
Myb controls G(2)/M progression by inducing cyclin B expression in the Drosophila eye imaginal disc.
|
| ジャーナル |
EMBO J
|
| Abstract |
The c-myb proto-oncogene product (c-Myb) is a transcriptional activator. Vertebrate c-Myb is a key regulator of the G(1)/S transition in cell cycle, while Drosophila Myb (dMyb) is important for the G(2)/M transition. Here we report that dMyb induces expression of cyclin B, a critical regulator of the G(2)/M transition, in Drosophila eye imaginal disc. In the wild-type eye disc, dmyb mRNA was expressed in the stripes both anterior and posterior to the morphogenetic furrow. Ectopic expression of C-terminal-truncated dMyb in the eye disc caused ectopic expression of cyclin B and the rough eye phenotype. This rough eye phenotype correlated with prolonged M phase, caused by overexpression of cyclin B. Cyclin B expression was lost in dmyb-deficient clones. In Schneider cells, the activity of the cyclin B promoter was dramatically reduced by loss of dMyb using the RNA interference method. Mutations of the multiple AACNG sequences in the cyclin B promoter also abolished the promoter activity. These results indicate that dMyb regulates the G(2)/M transition by inducing cyclin B expression via binding to its promoter.
|
| 巻・号 |
21(4)
|
| ページ |
675-84
|
| 公開日 |
2002-2-15
|
| DOI |
10.1093/emboj/21.4.675
|
| PMID |
11847115
|
| PMC |
PMC125351
|
| MeSH |
Animals
Animals, Genetically Modified
Base Sequence
Cyclin B / genetics*
DNA Primers
Drosophila
Drosophila Proteins
Eye / metabolism*
Eye / ultrastructure
G2 Phase / physiology*
Gene Expression Regulation / physiology*
Microscopy, Electron, Scanning
Mitosis / physiology*
Proto-Oncogene Proteins c-myb / physiology*
Reverse Transcriptase Polymerase Chain Reaction
Transcriptional Activation / physiology
|
| IF |
9.889
|
| 引用数 |
59
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
DGRC#109167 |
