RRC ID 30461
Author Jayaramaiah Raja S, Renkawitz-Pohl R.
Title Replacement by Drosophila melanogaster protamines and Mst77F of histones during chromatin condensation in late spermatids and role of sesame in the removal of these proteins from the male pronucleus.
Journal Mol. Cell. Biol.
Abstract Chromatin condensation is a typical feature of sperm cells. During mammalian spermiogenesis, histones are first replaced by transition proteins and then by protamines, while little is known for Drosophila melanogaster. Here we characterize three genes in the fly genome, Mst35Ba, Mst35Bb, and Mst77F. The results indicate that Mst35Ba and Mst35Bb encode dProtA and dProtB, respectively. These are considerably larger than mammalian protamines, but, as in mammals, both protamines contain typical cysteine/arginine clusters. Mst77F encodes a linker histone-like protein showing significant similarity to mammalian HILS1 protein. ProtamineA-enhanced green fluorescent protein (eGFP), ProtamineB-eGFP, and Mst77F-eGFP carrying Drosophila lines show that these proteins become the important chromosomal protein components of elongating spermatids, and His2AvDGFP vanishes. Mst77F mutants [ms(3)nc3] are characterized by small round nuclei and are sterile as males. These data suggest the major features of chromatin condensation in Drosophila spermatogenesis correspond to those in mammals. During early fertilization steps, the paternal pronucleus still contains protamines and Mst77F but regains a nucleosomal conformation before zygote formation. In eggs laid by sesame-deficient females, the paternal pronucleus remains in a protamine-based chromatin status but Mst77F-eGFP is removed, suggesting that the sesame gene product is essential for removal of protamines while Mst77F removal is independent of Sesame.
Volume 25(14)
Pages 6165-77
Published 2005-7
DOI 10.1128/MCB.25.14.6165-6177.2005
PII 25/14/6165
PMID 15988027
PMC PMC1168805
MeSH Amino Acid Sequence Animals Cell Cycle Proteins Cell Nucleus / metabolism Chromatin / genetics Chromatin / metabolism* Chromatin / physiology Chromosomal Proteins, Non-Histone Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila Proteins / physiology* Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Female Histone Chaperones Histones / genetics Histones / metabolism* Infertility, Male / genetics Male Molecular Sequence Data Mutation Protamines / genetics Protamines / metabolism* Spermatids / metabolism* Spermatogenesis / genetics Spermatogenesis / physiology* Transcription Factors Transcription, Genetic
IF 3.813
Resource
Drosophila DGRC#109173