論文 - 詳細
| RRC ID | 30507 |
|---|---|
| 著者 | Ishii H, Shirai T, Makino C, Nishikata T. |
| タイトル | Mitochondrial inhibitor sodium azide inhibits the reorganization of mitochondria-rich cytoplasm and the establishment of the anteroposterior axis in ascidian embryo. |
| ジャーナル | Dev Growth Differ |
| Abstract |
In ascidian eggs, cytoplasmic and cortical reorganization, previously called ooplasmic segregation, occurs in two phases during the first cell cycle. In the second phase of reorganization, the mitochondria-rich cytoplasm (myoplasm) moves to the future posterior side, concurrent with sperm aster migration along the egg cortex. Although this reorganization is the critical step for establishing the anteroposterior axis, its molecular mechanism is not fully understood. In this study, we showed that low concentrations of the mitochondrial inhibitor sodium azide (NaN3 ), which showed the low toxicity in sperm, inhibited the second phase of reorganization without the microtubule depolymerization. In the NaN3 -treated embryo, the sperm aster was not attracted to the cortex and altered its migration pathway; therefore, the myoplasm remained at the vegetal pole. Consequently, the anteroposterior axis was not established. Another mitochondrial inhibitor, oligomycin, did not affect these processes. These results suggest that NaN3 inhibits unknown molecules that are important for the second phase of reorganization. Identifying the target molecule of NaN3 will lead to a molecular understanding of cytoplasmic and cortical reorganization. |
| 巻・号 | 56(2) |
| ページ | 175-88 |
| 公開日 | 2014-2-1 |
| DOI | 10.1111/dgd.12117 |
| PMID | 24417477 |
| MeSH | Animals Body Patterning / drug effects Body Patterning / physiology* Cell Movement / drug effects Cleavage Stage, Ovum / drug effects Cleavage Stage, Ovum / physiology* Cytoplasm / drug effects* Male Mitochondria / drug effects* Phalloidine Sodium Azide / pharmacology* Sperm Motility / physiology Spermatozoa / drug effects Spermatozoa / physiology Urochordata / embryology* |
| IF | 1.723 |
| 引用数 | 7 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |