論文 - 詳細
| RRC ID | 30565 |
|---|---|
| 著者 | Kiuchi T, Koga H, Kawamoto M, Shoji K, Sakai H, Arai Y, Ishihara G, Kawaoka S, Sugano S, Shimada T, Suzuki Y, Suzuki MG, Katsuma S. |
| タイトル | A single female-specific piRNA is the primary determiner of sex in the silkworm. |
| ジャーナル | Nature |
| Abstract |
The silkworm Bombyx mori uses a WZ sex determination system that is analogous to the one found in birds and some reptiles. In this system, males have two Z sex chromosomes, whereas females have Z and W sex chromosomes. The silkworm W chromosome has a dominant role in female determination, suggesting the existence of a dominant feminizing gene in this chromosome. However, the W chromosome is almost fully occupied by transposable element sequences, and no functional protein-coding gene has been identified so far. Female-enriched PIWI-interacting RNAs (piRNAs) are the only known transcripts that are produced from the sex-determining region of the W chromosome, but the function(s) of these piRNAs are unknown. Here we show that a W-chromosome-derived, female-specific piRNA is the feminizing factor of B. mori. This piRNA is produced from a piRNA precursor which we named Fem. Fem sequences were arranged in tandem in the sex-determining region of the W chromosome. Inhibition of Fem-derived piRNA-mediated signalling in female embryos led to the production of the male-specific splice variants of B. mori doublesex (Bmdsx), a gene which acts at the downstream end of the sex differentiation cascade. A target gene of Fem-derived piRNA was identified on the Z chromosome of B. mori. This gene, which we named Masc, encoded a CCCH-type zinc finger protein. We show that the silencing of Masc messenger RNA by Fem piRNA is required for the production of female-specific isoforms of Bmdsx in female embryos, and that Masc protein controls both dosage compensation and masculinization in male embryos. Our study characterizes a single small RNA that is responsible for primary sex determination in the WZ sex determination system. |
| 巻・号 | 509(7502) |
| ページ | 633-6 |
| 公開日 | 2014-5-29 |
| DOI | 10.1038/nature13315 |
| PII | nature13315 |
| PMID | 24828047 |
| MeSH | Alternative Splicing / genetics Animals Base Sequence Bombyx / embryology Bombyx / genetics* Dosage Compensation, Genetic Female Male Molecular Sequence Data RNA, Small Interfering / genetics* Sex Characteristics* Sex Chromosomes / genetics Sex Determination Processes / genetics* |
| IF | 42.779 |
| 引用数 | 195 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 各媒体での言及数の合計 | 54 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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