RRC ID 30587
著者 Iitsuka T, Mita K, Hozumi A, Hamada M, Satoh N, Sasakura Y.
タイトル Transposon-mediated targeted and specific knockdown of maternally expressed transcripts in the ascidian Ciona intestinalis.
ジャーナル Sci Rep
Abstract Maternal mRNAs play crucial roles during early embryogenesis of ascidians, but their functions are largely unknown. In this study, we developed a new method to specifically knockdown maternal mRNAs in Ciona intestinalis using transposon-mediated transgenesis. We found that GFP expression is epigenetically silenced in Ciona intestinalis oocytes and eggs, and this epigenetic silencing of GFP was used to develop the knockdown method. When the 5' upstream promoter and 5' untranslated region (UTR) of a maternal gene are used to drive GFP in eggs, the maternal gene is specifically knocked down together with GFP. The 5' UTR of the maternal gene is the major element that determines the target gene silencing. Zygotic transcription of the target gene is unaffected, suggesting that the observed phenotypes specifically reflect the maternal function of the gene. This new method can provide breakthroughs in studying the functions of maternal mRNAs.
巻・号 4
ページ 5050
公開日 2014-5-24
DOI 10.1038/srep05050
PII srep05050
PMID 24854849
PMC PMC4031475
MeSH 5' Untranslated Regions / genetics* Animals Animals, Genetically Modified / genetics* Animals, Genetically Modified / growth & development Ciona intestinalis / genetics* Ciona intestinalis / growth & development DNA Methylation DNA Transposable Elements / genetics* Egg Proteins / antagonists & inhibitors Egg Proteins / genetics* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Female Gene Expression Regulation, Developmental Gene Silencing* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism In Situ Hybridization RNA Stability RNA, Messenger / antagonists & inhibitors RNA, Messenger / genetics*
IF 4.259
引用数 2
カタユウレイボヤ・(ニッポンウミシダ) Wild C. int Tg[MiCiTnIGCimTG]1 Tg[MiCiTnIGCipemG]2 Tg[MiCiTnIGCipemG]9