RRC ID 306
著者 Nishinokubi I, Shimoda M, Kako K, Sakai T, Fukamizu A, Ishida N.
タイトル Highly conserved Drosophila ananassae timeless gene functions as a clock component in Drosophila melanogaster.
ジャーナル Gene
Abstract The behavior and physiology of Drosophila are subject to rhythms that are controlled by the circadian clock genes, period, timeless, clock and cycle, all of which are thought to participate in central pacemaker control. The molecular mechanism of rhythm in Drosophila has been studied in detail. However, rhythm and clock genes have mostly been analyzed in Drosophila melanogaster. To confirm whether the tim gene exists and works as a clock component in other Drosophila species, we cloned a tim homolog from Drosophila ananassae that shared 85.9% similarity with Drosophila melanogaster tim at the amino acid level. In addition, the PER interaction domains and NLS were highly conserved. Introduction of the D. ananassae tim homolog rescued the rhythm of the locomotor activity of about 44% of a population of D. melanogaster tim(01) flies. At the molecular level, hs-tim introduced not only TIM but PER oscillation in transgenic flies. These results indicate that the tim gene in D. ananassae functions as a component of the circadian clock in D. melanogaster.
巻・号 307
ページ 183-90
公開日 2003-3-27
DOI 10.1016/s0378-1119(03)00468-2
PII S0378111903004682
PMID 12706901
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Biological Clocks / genetics* Circadian Rhythm / genetics Conserved Sequence / genetics DNA / chemistry DNA / genetics DNA, Complementary / chemistry DNA, Complementary / genetics Drosophila / genetics* Drosophila / physiology Drosophila Proteins / genetics* Drosophila melanogaster / genetics* Drosophila melanogaster / physiology Gene Expression Genes, Insect / genetics Genetic Complementation Test Locomotion / genetics Locomotion / physiology Molecular Sequence Data Mutation Nuclear Proteins / genetics Period Circadian Proteins Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Species Specificity
IF 2.984
引用数 7
WOS 分野 GENETICS & HEREDITY
リソース情報
ショウジョウバエ DGRC#109591