RRC ID 30602
著者 Kobayashi K, Kamei Y, Kinoshita M, Czerny T, Tanaka M.
タイトル A heat-inducible CRE/LOXP gene induction system in medaka.
ジャーナル Genesis
Abstract We established three lines of transgenic medaka, a heat-shock element (HSE) monitor line (hse-GFP line), heat-inducible driver lines (hse-cre lines), and effector lines (gapdh-loxP[DsRed]-GFP lines). We employed these to comprehensively analyze gene induction at different time points in various tissues. These analyses demonstrate a good response of synthetic HSEs by heat treatment during embryogenesis and the mosaic gene induction by cre/loxP-mediated recombination, thus providing practical information regarding the feasibility of a heat-inducible cre/loxP-mediated system in medaka. We also activated recombination by local heat-treatment using a metal probe and an infrared laser. Our results collectively indicate that these lines allow us to perform lineage tracing and mosaic analysis and provide the platform to investigate gene functions at later developmental stage and adult.
巻・号 51(1)
ページ 59-67
公開日 2013-1-1
DOI 10.1002/dvg.22348
PMID 23019184
MeSH Animals Animals, Genetically Modified / genetics* Gene Targeting Glyceraldehyde-3-Phosphate Dehydrogenases / genetics Heat-Shock Proteins / genetics Hot Temperature* Integrases / genetics* Oryzias / genetics* Transcriptional Activation*
IF 1.76
引用数 3
WOS 分野 GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY
リソース情報
メダカ BAC Full length cDNA gapdh-loxP[DsRed]-GFP (TG922 TG923) hse-cre:mCherry (TG918 TG919 TG920 TG921) OK-Cab (MT830)