| RRC ID |
30682
|
| 著者 |
Zaidman-Rémy A, Poidevin M, Hervé M, Welchman DP, Paredes JC, Fahlander C, Steiner H, Mengin-Lecreulx D, Lemaitre B.
|
| タイトル |
Drosophila immunity: analysis of PGRP-SB1 expression, enzymatic activity and function.
|
| ジャーナル |
PLoS One
|
| Abstract |
Peptidoglycan is an essential and specific component of the bacterial cell wall and therefore is an ideal recognition signature for the immune system. Peptidoglycan recognition proteins (PGRPs) are conserved from insects to mammals and able to bind PGN (non-catalytic PGRPs) and, in some cases, to efficiently degrade it (catalytic PGRPs). In Drosophila, several non-catalytic PGRPs function as selective peptidoglycan receptors upstream of the Toll and Imd pathways, the two major signalling cascades regulating the systemic production of antimicrobial peptides. Recognition PGRPs specifically activate the Toll pathway in response to Lys-type peptidoglycan found in most Gram-positive bacteria and the Imd pathway in response to DAP-type peptidoglycan encountered in Gram-positive bacilli-type bacteria and in Gram-negative bacteria. Catalytic PGRPs on the other hand can potentially reduce the level of immune activation by scavenging peptidoglycan. In accordance with this, PGRP-LB and PGRP-SC1A/B/2 have been shown to act as negative regulators of the Imd pathway. In this study, we report a biochemical and genetic analysis of PGRP-SB1, a catalytic PGRP. Our data show that PGRP-SB1 is abundantly secreted into the hemolymph following Imd pathway activation in the fat body, and exhibits an enzymatic activity towards DAP-type polymeric peptidoglycan. We have generated a PGRP-SB1/2 null mutant by homologous recombination, but its thorough phenotypic analysis did not reveal any immune function, suggesting a subtle role or redundancy of PGRP-SB1/2 with other molecules. Possible immune functions of PGRP-SB1 are discussed.
|
| 巻・号 |
6(2)
|
| ページ |
e17231
|
| 公開日 |
2011-2-18
|
| DOI |
10.1371/journal.pone.0017231
|
| PMID |
21364998
|
| PMC |
PMC3041801
|
| MeSH |
Animals
Animals, Genetically Modified
Carbohydrate Sequence
Carrier Proteins / genetics*
Carrier Proteins / metabolism*
Carrier Proteins / physiology*
Catalysis
Drosophila / enzymology
Drosophila / genetics
Drosophila / immunology*
Drosophila / metabolism
Drosophila Proteins / metabolism
Drosophila Proteins / physiology
Enzyme Activation / genetics
Enzyme Activation / immunology
Escherichia coli / metabolism
Gene Expression Regulation, Enzymologic
Immunity, Innate / genetics
Immunity, Innate / physiology*
Lactobacillus casei / metabolism
Models, Biological
Molecular Sequence Data
Myogenic Regulatory Factors / metabolism
Myogenic Regulatory Factors / physiology
Peptidoglycan / metabolism
Signal Transduction / immunology
Signal Transduction / physiology
Virulence Factors, Bordetella / metabolism
|
| IF |
2.74
|
| 引用数 |
54
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ショウジョウバエ |
|
