Reference - Detail
| RRC ID | 30705 |
|---|---|
| Author | Shimada A, Kawasoe Y, Hata Y, Takahashi TS, Masui R, Kuramitsu S, Fukui K. |
| Title | MutS stimulates the endonuclease activity of MutL in an ATP-hydrolysis-dependent manner. |
| Journal | FEBS J |
| Abstract |
In the initial steps of DNA mismatch repair, MutS recognizes a mismatched base and recruits the latent endonuclease MutL onto the mismatch-containing DNA in concert with other proteins. MutL then cleaves the error-containing strand to introduce an entry point for the downstream excision reaction. Because MutL has no intrinsic ability to recognize a mismatch and discriminate between newly synthesized and template strands, the endonuclease activity of MutL is strictly regulated by ATP-binding in order to avoid nonspecific degradation of the genomic DNA. However, the activation mechanism for its endonuclease activity remains unclear. In this study, we found that the coexistence of a mismatch, ATP and MutS unlocks the ATP-binding-dependent suppression of MutL endonuclease activity. Interestingly, ATPase-deficient mutants of MutS were unable to activate MutL. Furthermore, wild-type MutS activated ATPase-deficient mutants of MutL less efficiently than wild-type MutL. We concluded that ATP hydrolysis by MutS and MutL is involved in the mismatch-dependent activation of MutL endonuclease activity. |
| Volume | 280(14) |
| Pages | 3467-79 |
| Published | 2013-7-1 |
| DOI | 10.1111/febs.12344 |
| PMID | 23679952 |
| MeSH | Adenosine Triphosphatases / antagonists & inhibitors Adenosine Triphosphatases / chemistry Adenosine Triphosphatases / metabolism* Adenosine Triphosphate / chemistry* DNA Mismatch Repair Enzyme Activation Hydrolysis Kinetics MutS DNA Mismatch-Binding Protein / chemistry MutS DNA Mismatch-Binding Protein / metabolism* Plasmids / genetics Protein Structure, Tertiary Thermus thermophilus / enzymology* |
| IF | 4.392 |
| Times Cited | 13 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | Thermus thermophilus expression plasmid set TEx18D08 (THR007280) TEx18C04 (THR007252). |