論文 - 詳細
| RRC ID | 30731 |
|---|---|
| 著者 | Lim Y, Lee D, Kalichamy K, Hong SE, Michalak M, Ahnn J, Kim DH, Lee SK. |
| タイトル | Sumoylation regulates ER stress response by modulating calreticulin gene expression in XBP-1-dependent mode in Caenorhabditis elegans. |
| ジャーナル | Int J Biochem Cell Biol |
| Abstract |
Excessive accumulation of unfolded proteins in the endoplasmic reticulum (ER) lumen causes ER stress, which induces a set of genes, including those encoding ER-resident chaperones, to relieve the detrimental effects and recover homeostasis. Calreticulin is a chaperone that facilitates protein folding in the ER lumen, and its gene expression is induced by ER stress in Caenorhabditis elegans. Sumoylation conjugates small ubiquitin-like modifier (SUMO) proteins with target proteins to regulate a variety of biological processes, such as protein stability, nuclear transport, DNA binding, and gene expression. In this study, we showed that C. elegans X-box-binding protein 1 (Ce-XBP-1), an ER stress response transcription factor, interacts with the SUMO-conjugating enzyme UBC-9 and a SUMOylation target. Our results indicated that abolishing sumoylation enhanced calreticulin expression in an XBP-1-dependent manner, and the resulting increase in calreticulin counteracted ER stress. Furthermore, sumoylation was repressed in C. elegans undergoing ER stress. Finally, RNAi against ubc-9 mainly affected the expression of genes associated with ER functions, such as lipid and organic acid metabolism. Our results suggest that sumoylation plays a regulatory role in ER function by controlling the expression of genes required for ER homeostasis in C. elegans. |
| 巻・号 | 53 |
| ページ | 399-408 |
| 公開日 | 2014-8-1 |
| DOI | 10.1016/j.biocel.2014.06.005 |
| PII | S1357-2725(14)00203-9 |
| PMID | 24933177 |
| MeSH | Animals Caenorhabditis elegans Caenorhabditis elegans Proteins / biosynthesis* Calreticulin / biosynthesis* Carrier Proteins / biosynthesis* Endoplasmic Reticulum / genetics Endoplasmic Reticulum Stress / genetics* Gene Expression Regulation / genetics* Protein Folding Sumoylation / genetics Ubiquitin-Conjugating Enzymes / biosynthesis |
| IF | 3.673 |
| 引用数 | 12 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2610 |