| RRC ID |
30766
|
| Author |
Tominaga M, Kimura A, Yokota E, Haraguchi T, Shimmen T, Yamamoto K, Nakano A, Ito K.
|
| Title |
Cytoplasmic streaming velocity as a plant size determinant.
|
| Journal |
Dev Cell
|
| Abstract |
Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.
|
| Volume |
27(3)
|
| Pages |
345-52
|
| Published |
2013-11-11
|
| DOI |
10.1016/j.devcel.2013.10.005
|
| PII |
S1534-5807(13)00602-3
|
| PMID |
24229646
|
| MeSH |
Actins / genetics
Actins / metabolism*
Arabidopsis / growth & development*
Arabidopsis / metabolism*
Cell Size*
Cytoplasmic Streaming / physiology*
Immunoblotting
Myosins / genetics
Myosins / metabolism*
Plants, Genetically Modified
Protoplasts / metabolism
RNA, Messenger / genetics
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
10.092
|
| Times Cited |
57
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda01455 |
