論文 - 詳細
| RRC ID | 30772 |
|---|---|
| 著者 | Suematsu T, Watanabe O, Kita K, Yokobori S, Watanabe Y. |
| タイトル | Arabidopsis thaliana mitochondrial EF-G1 functions in two different translation steps. |
| ジャーナル | J Biochem |
| Abstract |
Translation elongation factor G (EF-G) in bacteria catalyses the translocation of transfer RNA on ribosomes in the elongation step as well as dissociation of post-termination state ribosomes into two subunits in the recycling step. In contrast, the dual functions of EF-G are exclusively divided into two different paralogues in human mitochondria, named EF-G1mt for translocation and EF-G2mt for ribosomal dissociation. Many of the two eukaryotic EF-G paralogues are phylogenetically associated with EF-G1mt and EF-G2mt groups. However, plant paralogues are associated with EF-G1mt and plastid EF-G, not with EF-G2mt. In this study, we phylogenetically and biochemically characterized Arabidopsis thaliana EF-G1mt (AtEF-G1mt) to clarify the factor responsible for the dissociation of ribosomes in plant mitochondria. We showed that eukaryotic EF-G1mts form one monophyletic group separated from bacterial EF-G and are classified into five sister groups. AtEF-G1mt is classified into a different group from its human counterpart. We also demonstrated that AtEF-G1mt catalyses both translocation and ribosomal dissociation, unlike in humans. Meanwhile, AtEF-G1mt is resistant to fusidic acid, an inhibitor of bacterial EF-G. Here, we propose that the functional division is not necessarily conserved among mitochondriate eukaryotes and also that EF-G1mt in organisms lacking EF-G2mt functions in two steps, similar to conventional bacterial EF-G. |
| 巻・号 | 155(2) |
| ページ | 107-14 |
| 公開日 | 2014-2-1 |
| DOI | 10.1093/jb/mvt105 |
| PII | mvt105 |
| PMID | 24272752 |
| MeSH | Arabidopsis / classification Arabidopsis / genetics Arabidopsis / metabolism* Evolution, Molecular Fusidic Acid / pharmacology GTP Phosphohydrolases / drug effects Humans Mitochondria / metabolism* Peptide Elongation Factor G / physiology* Phylogeny Protein Biosynthesis* Protein Synthesis Inhibitors / pharmacology |
| IF | 2.476 |
| 引用数 | 0 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda16175 |