Reference - Detail
| RRC ID | 30777 |
|---|---|
| Author | Mannen K, Matsumoto T, Takahashi S, Yamaguchi Y, Tsukagoshi M, Sano R, Suzuki H, Sakurai N, Shibata D, Koyama T, Nakayama T. |
| Title | Coordinated transcriptional regulation of isopentenyl diphosphate biosynthetic pathway enzymes in plastids by phytochrome-interacting factor 5. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
All isoprenoids are derived from a common C5 unit, isopentenyl diphosphate (IPP). In plants, IPP is synthesized via two distinct pathways; the cytosolic mevalonate pathway and the plastidial non-mevalonate (MEP) pathway. In this study, we used a co-expression analysis to identify transcription factors that coordinately regulate the expression of multiple genes encoding enzymes in the IPP biosynthetic pathway. Some candidates showed especially strong correlations with multiple genes encoding MEP-pathway enzymes. We report here that phytochrome-interacting factor 5 (PIF5), a basic-helix-loop-helix type transcription factor, functions as a positive regulator of the MEP pathway. Its overexpression in T87 suspension cultured cells resulted in increased accumulation of chlorophylls and carotenoids. Detailed analyses of carotenoids by HPLC indicated that some carotenoid biosynthetic pathways were concomitantly up-regulated, possibly as a result of enhanced IPP metabolic flow. Our results also revealed other PIF family proteins that play different roles from that of PIF5 in IPP metabolism. |
| Volume | 443(2) |
| Pages | 768-74 |
| Published | 2014-1-10 |
| DOI | 10.1016/j.bbrc.2013.12.040 |
| PII | S0006-291X(13)02099-8 |
| PMID | 24342623 |
| MeSH | Arabidopsis / genetics* Arabidopsis Proteins / genetics* Basic Helix-Loop-Helix Transcription Factors / genetics* Hemiterpenes / biosynthesis* Hemiterpenes / genetics Multienzyme Complexes / genetics* Multienzyme Complexes / metabolism Organophosphorus Compounds Plastids / genetics* Signal Transduction / genetics Transcription Factors / genetics* Transcriptional Activation / genetics* |
| IF | 2.985 |
| Times Cited | 12 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00008 |