| RRC ID |
30781
|
| Author |
Kansup J, Tsugama D, Liu S, Takano T.
|
| Title |
Arabidopsis G-protein β subunit AGB1 interacts with NPH3 and is involved in phototropism.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Heterotrimeric G proteins (Gα, Gβ and Gγ) have pleiotropic roles in plants, but molecular mechanisms underlying them remain to be elucidated. Here we show that Arabidopsis Gβ (AGB1) interacts with NPH3, a regulator of phototropism. Yeast two-hybrid assays, in vitro pull-down assays and bimolecular fluorescence complementation assays showed that AGB1 and NPH3 physically interact. NPH3-null mutation (nph3) is known to completely abolish hypocotyl phototropism. Loss-of-function mutants of AGB1 (agb1-1 and agb1-2) showed decreased hypocotyl phototropism, and agb1/nph3 double mutants showed no hypocotyl phototropism. These results suggest that AGB1 is involved in the NPH3-mediated regulation of phototropism.
|
| Volume |
445(1)
|
| Pages |
54-7
|
| Published |
2014-2-28
|
| DOI |
10.1016/j.bbrc.2014.01.106
|
| PII |
S0006-291X(14)00141-7
|
| PMID |
24486545
|
| MeSH |
Arabidopsis / genetics
Arabidopsis / metabolism
Arabidopsis / physiology*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Blotting, Western
GTP-Binding Protein beta Subunits / genetics
GTP-Binding Protein beta Subunits / metabolism*
Gene Expression Regulation, Plant
Hypocotyl / genetics
Hypocotyl / metabolism
Hypocotyl / physiology
Mutation
Phototropism / genetics
Phototropism / physiology*
Protein Binding
Reverse Transcriptase Polymerase Chain Reaction
Two-Hybrid System Techniques
|
| IF |
2.985
|
| Times Cited |
9
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda18592 |
