RRC ID 308
Author Shirai T, Maehara A, Kiritooshi N, Matsuzaki F, Handa H, Nakagoshi H.
Title Differential requirement of EGFR signaling for the expression of defective proventriculus gene in the Drosophila endoderm and ectoderm.
Journal Biochem. Biophys. Res. Commun.
Abstract A homeobox gene, defective proventriculus (dve), is expressed in various tissues including the ventral ectoderm and midgut. Here, we show the expression pattern of dve in the ventral ectoderm, in which dve expression is induced by Spitz, a ligand for Drosophila epidermal growth factor receptor (EGFR). In spitz mutants, dve expression is only lost in the ventral ectoderm and overexpression of Spitz induces ectopic dve activation in the ventral ectoderm. Dve expression in the middle midgut depends on Decapentaplegic (Dpp) signaling, while expression of a dominant-negative form of Drosophila EGFR (DER(DN)) also causes a marked decrease in dve expression in the middle midgut. Furthermore, heterozygous mutation of thick veins (tkv), a Dpp receptor, strongly enhances the effect of DER(DN). These results indicate that EGFR signaling is crucial for dve expression in the ventral ectoderm and is required in the middle midgut where it cooperates with Dpp signaling.
Volume 311(2)
Pages 473-7
Published 2003-11-14
PII S0006291X03020874
PMID 14592438
MeSH Animals Drosophila / embryology* Drosophila / genetics Drosophila / metabolism* Drosophila Proteins* Ectoderm / metabolism* Endoderm / metabolism* Epidermal Growth Factor* ErbB Receptors / genetics ErbB Receptors / metabolism* Gene Expression Regulation, Developmental / physiology* Homeodomain Proteins / genetics Homeodomain Proteins / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism Tissue Distribution
IF 2.559
Times Cited 7