| RRC ID |
3082
|
| 著者 |
Calderón-Villalobos LI, Nill C, Marrocco K, Kretsch T, Schwechheimer C.
|
| タイトル |
The evolutionarily conserved Arabidopsis thaliana F-box protein AtFBP7 is required for efficient translation during temperature stress.
|
| ジャーナル |
Gene
|
| Abstract |
In eukaryotes, E3 ubiquitin ligases (E3s) mediate the ubiquitylation of proteins that are destined for degradation by the ubiquitin-proteasome system. In SKP1/CDC53/F-box protein (SCF)-type E3 complexes, the interchangeable F-box protein confers specificity to the E3 ligase through direct physical interactions with the degradation substrate. The vast majority of the approximately 700 F-box proteins from the plant model organism Arabidopsis thaliana remain to be characterized. Here, we investigate the previously uncharacterized and evolutionarily conserved Arabidopsis F-box protein 7 (AtFBP7), which is encoded by a unique gene in Arabidopsis (At1g21760). Several apparent fbp7 loss-of-function alleles do not have an obvious phenotype. AtFBP7 is ubiquitously expressed and its expression is induced after cold and heat stress. When following up on a reported co-purification of the eukaryotic elongation factor-2 (eEF-2) with YLR097c, the apparent budding yeast orthologue of AtFBP7, we discovered a general defect in protein biosynthesis after cold and heat stress in fbp7 mutants. Thus, our findings suggest that AtFBP7 is required for protein synthesis during temperature stress.
|
| 巻・号 |
392(1-2)
|
| ページ |
106-16
|
| 公開日 |
2007-5-1
|
| DOI |
10.1016/j.gene.2006.11.016
|
| PII |
S0378-1119(06)00718-9
|
| PMID |
17240087
|
| MeSH |
Adaptation, Biological / genetics
Amino Acid Sequence
Arabidopsis / genetics*
Arabidopsis / metabolism
Conserved Sequence
Evolution, Molecular
F-Box Proteins / genetics*
F-Box Proteins / metabolism
F-Box Proteins / physiology*
Molecular Sequence Data
Phylogeny
Protein Biosynthesis*
Sequence Homology, Amino Acid
Temperature*
Tissue Distribution
|
| IF |
2.984
|
| 引用数 |
30
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pst17753
pst18526
pst19185 |
