論文 - 詳細
| RRC ID | 3082 |
|---|---|
| 著者 | Calderón-Villalobos LI, Nill C, Marrocco K, Kretsch T, Schwechheimer C. |
| タイトル | The evolutionarily conserved Arabidopsis thaliana F-box protein AtFBP7 is required for efficient translation during temperature stress. |
| ジャーナル | Gene |
| Abstract |
In eukaryotes, E3 ubiquitin ligases (E3s) mediate the ubiquitylation of proteins that are destined for degradation by the ubiquitin-proteasome system. In SKP1/CDC53/F-box protein (SCF)-type E3 complexes, the interchangeable F-box protein confers specificity to the E3 ligase through direct physical interactions with the degradation substrate. The vast majority of the approximately 700 F-box proteins from the plant model organism Arabidopsis thaliana remain to be characterized. Here, we investigate the previously uncharacterized and evolutionarily conserved Arabidopsis F-box protein 7 (AtFBP7), which is encoded by a unique gene in Arabidopsis (At1g21760). Several apparent fbp7 loss-of-function alleles do not have an obvious phenotype. AtFBP7 is ubiquitously expressed and its expression is induced after cold and heat stress. When following up on a reported co-purification of the eukaryotic elongation factor-2 (eEF-2) with YLR097c, the apparent budding yeast orthologue of AtFBP7, we discovered a general defect in protein biosynthesis after cold and heat stress in fbp7 mutants. Thus, our findings suggest that AtFBP7 is required for protein synthesis during temperature stress. |
| 巻・号 | 392(1-2) |
| ページ | 106-16 |
| 公開日 | 2007-5-1 |
| DOI | 10.1016/j.gene.2006.11.016 |
| PII | S0378-1119(06)00718-9 |
| PMID | 17240087 |
| MeSH | Adaptation, Biological / genetics Amino Acid Sequence Arabidopsis / genetics* Arabidopsis / metabolism Conserved Sequence Evolution, Molecular F-Box Proteins / genetics* F-Box Proteins / metabolism F-Box Proteins / physiology* Molecular Sequence Data Phylogeny Protein Biosynthesis* Sequence Homology, Amino Acid Temperature* Tissue Distribution |
| IF | 2.984 |
| 引用数 | 30 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst17753 pst18526 pst19185 |