Reference - Detail
RRC ID | 3086 |
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Author | Koyama T, Furutani M, Tasaka M, Ohme-Takagi M. |
Title | TCP transcription factors control the morphology of shoot lateral organs via negative regulation of the expression of boundary-specific genes in Arabidopsis. |
Journal | Plant Cell |
Abstract |
Plants form shoot meristems in the so-called boundary region, and these meristems are necessary for normal morphogenesis of aerial parts of plants. However, the molecular mechanisms that regulate the formation of shoot meristems are not fully understood. We report here that expression of a chimeric repressor from TCP3 (TCP3SRDX), a member of TEOSINTE BRANCHED1, CYCLOIDEA, and PCF (TCP) transcription factors in Arabidopsis thaliana, resulted in the formation of ectopic shoots on cotyledons and various defects in organ development. Expression of TCP3SRDX induced ectopic expression of boundary-specific genes, namely the CUP-SHAPED COTYLEDON (CUC) genes, and suppressed the expression of miR164, whose product cleaves the transcripts of CUC genes. This abnormal phenotype was substantially reversed on the cuc1 mutant background. By contrast, gain of function of TCP3 suppressed the expression of CUC genes and resulted in the fusion of cotyledons and defects in formation of shoots. The pattern of expression of TCP3 did not overlap with that of the CUC genes. In addition, we found that eight TCPs had functions similar to that of TCP3. Our results demonstrate that the TCP transcription factors play a pivotal role in the control of morphogenesis of shoot organs by negatively regulating the expression of boundary-specific genes. |
Volume | 19(2) |
Pages | 473-84 |
Published | 2007-2-1 |
DOI | 10.1105/tpc.106.044792 |
PII | tpc.106.044792 |
PMID | 17307931 |
PMC | PMC1867346 |
MeSH | Arabidopsis* / anatomy & histology Arabidopsis* / genetics Arabidopsis* / growth & development Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Gene Expression Regulation, Developmental* Gene Expression Regulation, Plant* In Situ Hybridization Meristem / anatomy & histology Meristem / growth & development Molecular Sequence Data Morphogenesis* Phenotype Plant Shoots* / anatomy & histology Plant Shoots* / growth & development Plants, Genetically Modified Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism* |
IF | 9.618 |
Times Cited | 241 |
WOS Category | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
Resource | |
Arabidopsis / Cultured plant cells, genes | pda01673 pda01786 pda07585 pda08666 pda12415 |