RRC ID 30925
Author Daido Y, Hamanishi S, Kusakabe TG.
Title Transcriptional co-regulation of evolutionarily conserved microRNA/cone opsin gene pairs: implications for photoreceptor subtype specification.
Journal Dev. Biol.
Abstract The vertebrate retina contains two types of photoreceptor cells, rods and cones, which use distinct types of opsins and phototransduction proteins. Cones can be further divided into several subtypes with differing wavelength sensitivity and morphology. Although photoreceptor development has been extensively studied in a variety of vertebrate species, the mechanism by which photoreceptor subtypes are established is still largely unknown. Here we report two microRNAs (miRNAs), miR-726 and miR-729, which are potentially involved in photoreceptor subtype specification. In the medaka Oryzias latipes, the genes encoding miR-726 and miR-729 are located upstream of the red-sensitive opsin gene LWS-A and the UV-sensitive opsin gene SWS1, respectively, and are transcribed in the opposite direction from the respective opsin genes. The miR-726/LWS pair is conserved between teleosts and tetrapods, and the miR-729/SWS1 pair is conserved among teleosts. in situ hybridization analyses and fluorescence reporter assays suggest that these miRNAs are co-expressed with the respective opsins in specific cone subtypes. Potential targets of miR-726 and miR-729 predicted in silico include several transcription factors that regulate photoreceptor development. Functional analyses of cis-regulatory sequences in vivo suggest that transcription of the paired microRNA and opsin genes is co-regulated by common cis-regulatory modules. We propose an evolutionarily conserved mechanism that controls photoreceptor subtype identity through coupling between transcriptional and post-transcriptional regulations.
Volume 392(1)
Pages 117-29
Published 2014-8-1
DOI 10.1016/j.ydbio.2014.04.021
PII S0012-1606(14)00239-5
PMID 24797636
MeSH Animals Base Sequence Cone Opsins / biosynthesis Cone Opsins / genetics* Conserved Sequence / genetics Evolution, Molecular* Gene Expression Regulation MicroRNAs / biosynthesis MicroRNAs / genetics* Oryzias / genetics* Photoreceptor Cells, Vertebrate Retina / cytology Retina / physiology Retinal Cone Photoreceptor Cells / classification* Sequence Alignment Sequence Analysis, DNA Transcription, Genetic
IF 3.262
Times Cited 4
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Medaka Hatching enzyme