論文 - 詳細
| RRC ID | 30925 |
|---|---|
| 著者 | Daido Y, Hamanishi S, Kusakabe TG. |
| タイトル | Transcriptional co-regulation of evolutionarily conserved microRNA/cone opsin gene pairs: implications for photoreceptor subtype specification. |
| ジャーナル | Dev Biol |
| Abstract |
The vertebrate retina contains two types of photoreceptor cells, rods and cones, which use distinct types of opsins and phototransduction proteins. Cones can be further divided into several subtypes with differing wavelength sensitivity and morphology. Although photoreceptor development has been extensively studied in a variety of vertebrate species, the mechanism by which photoreceptor subtypes are established is still largely unknown. Here we report two microRNAs (miRNAs), miR-726 and miR-729, which are potentially involved in photoreceptor subtype specification. In the medaka Oryzias latipes, the genes encoding miR-726 and miR-729 are located upstream of the red-sensitive opsin gene LWS-A and the UV-sensitive opsin gene SWS1, respectively, and are transcribed in the opposite direction from the respective opsin genes. The miR-726/LWS pair is conserved between teleosts and tetrapods, and the miR-729/SWS1 pair is conserved among teleosts. in situ hybridization analyses and fluorescence reporter assays suggest that these miRNAs are co-expressed with the respective opsins in specific cone subtypes. Potential targets of miR-726 and miR-729 predicted in silico include several transcription factors that regulate photoreceptor development. Functional analyses of cis-regulatory sequences in vivo suggest that transcription of the paired microRNA and opsin genes is co-regulated by common cis-regulatory modules. We propose an evolutionarily conserved mechanism that controls photoreceptor subtype identity through coupling between transcriptional and post-transcriptional regulations. |
| 巻・号 | 392(1) |
| ページ | 117-29 |
| 公開日 | 2014-8-1 |
| DOI | 10.1016/j.ydbio.2014.04.021 |
| PII | S0012-1606(14)00239-5 |
| PMID | 24797636 |
| MeSH | Animals Base Sequence Cone Opsins / biosynthesis Cone Opsins / genetics* Conserved Sequence / genetics Evolution, Molecular* Gene Expression Regulation MicroRNAs / biosynthesis MicroRNAs / genetics* Oryzias / genetics* Photoreceptor Cells, Vertebrate Retina / cytology Retina / physiology Retinal Cone Photoreceptor Cells / classification* Sequence Alignment Sequence Analysis, DNA Transcription, Genetic |
| IF | 2.896 |
| 引用数 | 7 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| メダカ | Hatching enzyme |