RRC ID 30929
Author Kumazaki K, Tsukazaki T, Nishizawa T, Tanaka Y, Kato HE, Nakada-Nakura Y, Hirata K, Mori Y, Suga H, Dohmae N, Ishitani R, Nureki O.
Title Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans.
Journal Acta Crystallogr F Struct Biol Commun
Abstract YidC, a member of the YidC/Oxa1/Alb3 family, inserts proteins into the membrane and facilitates membrane-protein folding in bacteria. YidC plays key roles in both Sec-mediated integration and Sec-independent insertion of membrane proteins. Here, Bacillus halodurans YidC2, which has five transmembrane helices conserved among the other family members, was identified as a target protein for structure determination by a fluorescent size-exclusion chromatography analysis. The protein was overexpressed, purified and crystallized in the lipidic cubic phase. The crystals diffracted X-rays to 2.4 Å resolution and belonged to space group P21, with unit-cell parameters a = 43.9, b = 60.6, c = 58.9 Å, β = 100.3°. The experimental phases were determined by the multiwavelength anomalous diffraction method using a mercury-derivatized crystal.
Volume 70(Pt 8)
Pages 1056-60
Published 2014-8
DOI 10.1107/S2053230X14012540
PII S2053230X14012540
PMID 25084381
PMC PMC4118803
MeSH Bacillus / chemistry* Bacillus / enzymology Bacterial Proteins / chemistry* Chromatography, Gel Crystallography, X-Ray Enzymes / chemistry* Molecular Chaperones / chemistry*
IF 0.989
Times Cited 2
WOS Category CRYSTALLOGRAPHY BIOCHEMICAL RESEARCH METHODS BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material Genomic DNA of Bacillus halodurans JCM 9153 (JGD12232)
General Microbes