| RRC ID |
30929
|
| Author |
Kumazaki K, Tsukazaki T, Nishizawa T, Tanaka Y, Kato HE, Nakada-Nakura Y, Hirata K, Mori Y, Suga H, Dohmae N, Ishitani R, Nureki O.
|
| Title |
Crystallization and preliminary X-ray diffraction analysis of YidC, a membrane-protein chaperone and insertase from Bacillus halodurans.
|
| Journal |
Acta Crystallogr F Struct Biol Commun
|
| Abstract |
YidC, a member of the YidC/Oxa1/Alb3 family, inserts proteins into the membrane and facilitates membrane-protein folding in bacteria. YidC plays key roles in both Sec-mediated integration and Sec-independent insertion of membrane proteins. Here, Bacillus halodurans YidC2, which has five transmembrane helices conserved among the other family members, was identified as a target protein for structure determination by a fluorescent size-exclusion chromatography analysis. The protein was overexpressed, purified and crystallized in the lipidic cubic phase. The crystals diffracted X-rays to 2.4 Å resolution and belonged to space group P21, with unit-cell parameters a = 43.9, b = 60.6, c = 58.9 Å, β = 100.3°. The experimental phases were determined by the multiwavelength anomalous diffraction method using a mercury-derivatized crystal.
|
| Volume |
70(Pt 8)
|
| Pages |
1056-60
|
| Published |
2014-8-1
|
| DOI |
10.1107/S2053230X14012540
|
| PII |
S2053230X14012540
|
| PMID |
25084381
|
| PMC |
PMC4118803
|
| MeSH |
Bacillus / chemistry*
Bacillus / enzymology
Bacterial Proteins / chemistry*
Chromatography, Gel
Crystallography, X-Ray
Enzymes / chemistry*
Molecular Chaperones / chemistry*
|
| IF |
0.968
|
| Times Cited |
5
|
|
WOS Category
|
CRYSTALLOGRAPHY
BIOCHEMICAL RESEARCH METHODS
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
Genomic DNA of Bacillus halodurans JCM 9153 (JGD12232) |
| General Microbes |
|
