論文 - 詳細
| RRC ID | 30932 |
|---|---|
| 著者 | Takano K, Aoi A, Koga Y, Kanaya S. |
| タイトル | Evolvability of thermophilic proteins from archaea and bacteria. |
| ジャーナル | Biochemistry |
| Abstract |
Proteins from thermophiles possess high thermostability. The stabilization mechanisms differ between archaeal and bacterial proteins, whereby archaeal proteins are mainly stabilized via hydrophobic interactions and bacterial proteins by ion pairs. High stability is an important factor in promoting protein evolution, but the precise means by which different stabilization mechanisms affect the evolution process remain unclear. In this study, we investigated a random mutational drift of esterases from thermophilic archaea and bacteria at high temperatures. Our results indicate that mutations in archaeal proteins lead to improved function with no loss of stability, while mutant bacterial proteins are largely destabilized with decreased activity at high temperatures. On the basis of these findings, we suggest that archaeal proteins possess higher "evolvability" than bacterial proteins under temperature selection and are additionally able to evolve into eukaryotic proteins. |
| 巻・号 | 52(28) |
| ページ | 4774-80 |
| 公開日 | 2013-7-16 |
| DOI | 10.1021/bi400652c |
| PMID | 23822830 |
| MeSH | Archaeal Proteins / chemistry* Archaeal Proteins / genetics Bacterial Proteins / chemistry* Bacterial Proteins / genetics Directed Molecular Evolution* Models, Molecular Polymerase Chain Reaction Temperature |
| IF | 2.865 |
| 引用数 | 13 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| 遺伝子材料 | Genomic DNA of Thermotoga maritima JCM 10099T (JGD07695) |
| 一般微生物 | |