論文 - 詳細
| RRC ID | 30935 |
|---|---|
| 著者 | Niwa H, Yamamura K, Miyazaki J. |
| タイトル | Efficient selection for high-expression transfectants with a novel eukaryotic vector. |
| ジャーナル | Gene |
| Abstract |
We have developed a new expression vector which allows efficient selection for transfectants that express foreign genes at high levels. The vector is composed of a ubiquitously strong promoter based on the beta-actin promoter, a 69% subregion of the bovine papilloma virus genome, and a mutant neomycin phosphotransferase II-encoding gene driven by a weak promoter, which confers only marginal resistance to G418. Thus, high concentrations of G418 (approx. 800 micrograms/ml) effectively select for transfectants containing a high vector copy number (greater than 300). We tested this system by producing human interleukin-2 (IL-2) in L cells and Chinese hamster ovary (CHO) cells, and the results showed that high concentrations of G418 efficiently yielded L cell and CHO cell transfectants stably producing IL-2 at levels comparable with those previously attained using gene amplification. The vector sequences were found to have integrated into the host chromosome, and were stably maintained in the transfectants for several months. |
| 巻・号 | 108(2) |
| ページ | 193-9 |
| 公開日 | 1991-12-15 |
| DOI | 10.1016/0378-1119(91)90434-d |
| PII | 0378-1119(91)90434-D |
| PMID | 1660837 |
| MeSH | Actins / genetics Animals Blotting, Northern Blotting, Southern Bovine papillomavirus 1 / genetics* CHO Cells Cattle Cricetinae Genetic Vectors / genetics* Gentamicins / pharmacology Humans Interleukin-2 / genetics Kanamycin Kinase L Cells Mice Phosphotransferases / genetics Promoter Regions, Genetic / genetics Recombinant Proteins / biosynthesis* Recombinant Proteins / genetics Selection, Genetic Transfection / genetics beta-Galactosidase / genetics beta-Galactosidase / metabolism |
| IF | 2.984 |
| 引用数 | 4324 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 642 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 12.0 |
| リソース情報 | |
| 遺伝子材料 | pCAGGS (RDB08938) |