論文 - 詳細
| RRC ID | 30941 |
|---|---|
| 著者 | Ishii J, Kondo T, Makino H, Ogura A, Matsuda F, Kondo A. |
| タイトル | Three gene expression vector sets for concurrently expressing multiple genes in Saccharomyces cerevisiae. |
| ジャーナル | FEMS Yeast Res |
| Abstract |
Yeast has the potential to be used in bulk-scale fermentative production of fuels and chemicals due to its tolerance for low pH and robustness for autolysis. However, expression of multiple external genes in one host yeast strain is considerably labor-intensive due to the lack of polycistronic transcription. To promote the metabolic engineering of yeast, we generated systematic and convenient genetic engineering tools to express multiple genes in Saccharomyces cerevisiae. We constructed a series of multi-copy and integration vector sets for concurrently expressing two or three genes in S. cerevisiae by embedding three classical promoters. The comparative expression capabilities of the constructed vectors were monitored with green fluorescent protein, and the concurrent expression of genes was monitored with three different fluorescent proteins. Our multiple gene expression tool will be helpful to the advanced construction of genetically engineered yeast strains in a variety of research fields other than metabolic engineering. |
| 巻・号 | 14(3) |
| ページ | 399-411 |
| 公開日 | 2014-5-1 |
| DOI | 10.1111/1567-1364.12138 |
| PMID | 24447461 |
| MeSH | Gene Expression* Genes, Reporter Genetic Vectors* Genetics, Microbial / methods* Luminescent Proteins / analysis Luminescent Proteins / genetics Metabolic Engineering / methods* Molecular Biology / methods* Promoter Regions, Genetic Recombinant Proteins / analysis Recombinant Proteins / genetics Saccharomyces cerevisiae / genetics* |
| IF | 3.193 |
| 引用数 | 24 |
| WOS 分野 | MYCOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BYP7579 BYP7580 BYP7581 BYP7582 BYP7583 BYP7584 BYP7585 BYP7586 BYP7587 BYP7588 |