RRC ID 30950
Author Fischer M, Haase I, Simmeth E, Gerisch G, Müller-Taubenberger A.
Title A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium.
Journal FEBS Lett.
Abstract Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis of RFP from Discosoma (DsRed) resulted in the monomeric mRFP1 and in the tetrameric RedStar RFP, which is distinguished by its enhanced fluorescence in vivo. Based on these mutations, we have generated an enhanced monomeric RFP, mRFPmars, and report its spectral properties. Together with green fluorescent labels, we used mRFPmars to visualize filamentous actin structures and microtubules in Dictyostelium cells. This enhanced RFP proved to be suitable to monitor the dynamics of cytoskeletal proteins in cell motility, mitosis, and endocytosis using dual-wavelength fluorescence microscopy.
Volume 577(1-2)
Pages 227-32
Published 2004-11-5
DOI 10.1016/j.febslet.2004.09.084
PII S0014579304012414
PMID 15527790
MeSH Amino Acid Sequence Animals Base Sequence Cytoskeleton / ultrastructure* Dictyostelium / ultrastructure* Electrophoresis, Polyacrylamide Gel Endocytosis In Situ Hybridization, Fluorescence Luminescent Proteins / chemistry Luminescent Proteins / genetics Luminescent Proteins / metabolism* Molecular Sequence Data
IF 2.999
Times Cited 89
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Cellular slime molds G90005