Reference - Detail
| RRC ID | 30950 |
|---|---|
| Author | Fischer M, Haase I, Simmeth E, Gerisch G, Müller-Taubenberger A. |
| Title | A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium. |
| Journal | FEBS Lett |
| Abstract |
Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis of RFP from Discosoma (DsRed) resulted in the monomeric mRFP1 and in the tetrameric RedStar RFP, which is distinguished by its enhanced fluorescence in vivo. Based on these mutations, we have generated an enhanced monomeric RFP, mRFPmars, and report its spectral properties. Together with green fluorescent labels, we used mRFPmars to visualize filamentous actin structures and microtubules in Dictyostelium cells. This enhanced RFP proved to be suitable to monitor the dynamics of cytoskeletal proteins in cell motility, mitosis, and endocytosis using dual-wavelength fluorescence microscopy. |
| Volume | 577(1-2) |
| Pages | 227-32 |
| Published | 2004-11-5 |
| DOI | 10.1016/j.febslet.2004.09.084 |
| PII | S0014579304012414 |
| PMID | 15527790 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cytoskeleton / ultrastructure* Dictyostelium / ultrastructure* Electrophoresis, Polyacrylamide Gel Endocytosis In Situ Hybridization, Fluorescence Luminescent Proteins / chemistry Luminescent Proteins / genetics Luminescent Proteins / metabolism* Molecular Sequence Data |
| IF | 3.057 |
| Times Cited | 112 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 69 |
| Altmetric score changes over past 6months | 6.0 |
| Resource | |
| Cellular slime molds | G90005 |