| RRC ID |
30950
|
| 著者 |
Fischer M, Haase I, Simmeth E, Gerisch G, Müller-Taubenberger A.
|
| タイトル |
A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium.
|
| ジャーナル |
FEBS Lett
|
| Abstract |
Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis of RFP from Discosoma (DsRed) resulted in the monomeric mRFP1 and in the tetrameric RedStar RFP, which is distinguished by its enhanced fluorescence in vivo. Based on these mutations, we have generated an enhanced monomeric RFP, mRFPmars, and report its spectral properties. Together with green fluorescent labels, we used mRFPmars to visualize filamentous actin structures and microtubules in Dictyostelium cells. This enhanced RFP proved to be suitable to monitor the dynamics of cytoskeletal proteins in cell motility, mitosis, and endocytosis using dual-wavelength fluorescence microscopy.
|
| 巻・号 |
577(1-2)
|
| ページ |
227-32
|
| 公開日 |
2004-11-5
|
| DOI |
10.1016/j.febslet.2004.09.084
|
| PII |
S0014579304012414
|
| PMID |
15527790
|
| MeSH |
Amino Acid Sequence
Animals
Base Sequence
Cytoskeleton / ultrastructure*
Dictyostelium / ultrastructure*
Electrophoresis, Polyacrylamide Gel
Endocytosis
In Situ Hybridization, Fluorescence
Luminescent Proteins / chemistry
Luminescent Proteins / genetics
Luminescent Proteins / metabolism*
Molecular Sequence Data
|
| IF |
3.057
|
| 引用数 |
112
|
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| 細胞性粘菌 |
G90005 |
