RRC ID 30957
Author Takaine M, Imada K, Numata O, Nakamura T, Nakano K.
Title The meiosis-specific nuclear passenger protein is required for proper assembly of forespore membrane in fission yeast.
Journal J. Cell. Sci.
Abstract Sporulation, gametogenesis in yeast, consists of meiotic nuclear division and spore morphogenesis. In the fission yeast Schizosaccharomyces pombe, the four haploid nuclei produced after meiosis II are encapsulated by the forespore membrane (FSM), which is newly synthesized from spindle pole bodies (SPBs) in the cytoplasm of the mother cell as spore precursors. Although the coordination between meiosis and FSM assembly is vital for proper sporulation, the underlying mechanism remains unclear. In the present study, we identified a new meiosis-specific protein Npg1, and found that it was involved in the efficient formation of spores and spore viability. The accumulation and organization of the FSM was compromised in npg1-null cells, leading to the error-prone envelopment of nuclei. Npg1 was first seen as internuclear dots and translocated to the SPBs before the FSM assembled. Genetic analysis revealed that Npg1 worked in conjunction with the FSM proteins Spo3 and Meu14. These results suggest a possible signaling link from the nucleus to the meiotic SPBs in order to associate the onset of FSM assembly with meiosis II, which ensures the successful partitioning of gametic nuclei.
Volume 127(Pt 20)
Pages 4429-42
Published 2014-10-15
DOI 10.1242/jcs.151738
PII jcs.151738
PMID 25146394
MeSH Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Membrane / metabolism* Cell Nucleus / physiology* Gene Expression Regulation, Fungal / genetics Gene Knockout Techniques Meiosis / genetics Meiosis / physiology* Morphogenesis / genetics Protein Transport / genetics Saccharomyces cerevisiae Proteins / metabolism Schizosaccharomyces / physiology* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Signal Transduction Spindle Pole Bodies / metabolism* Spores, Fungal / genetics
IF 4.401
Times Cited 1
Yeast FY21373 FY21084 FY12450 FY21068 FY19919 FY15884 FY21086