RRC ID 30961
Author Van de Velde W, Zehirov G, Szatmari A, Debreczeny M, Ishihara H, Kevei Z, Farkas A, Mikulass K, Nagy A, Tiricz H, Satiat-Jeunemaître B, Alunni B, Bourge M, Kucho K, Abe M, Kereszt A, Maroti G, Uchiumi T, Kondorosi E, Mergaert P.
Title Plant peptides govern terminal differentiation of bacteria in symbiosis.
Journal Science
Abstract Legume plants host nitrogen-fixing endosymbiotic Rhizobium bacteria in root nodules. In Medicago truncatula, the bacteria undergo an irreversible (terminal) differentiation mediated by hitherto unidentified plant factors. We demonstrated that these factors are nodule-specific cysteine-rich (NCR) peptides that are targeted to the bacteria and enter the bacterial membrane and cytosol. Obstruction of NCR transport in the dnf1-1 signal peptidase mutant correlated with the absence of terminal bacterial differentiation. On the contrary, ectopic expression of NCRs in legumes devoid of NCRs or challenge of cultured rhizobia with peptides provoked symptoms of terminal differentiation. Because NCRs resemble antimicrobial peptides, our findings reveal a previously unknown innovation of the host plant, which adopts effectors of the innate immune system for symbiosis to manipulate the cell fate of endosymbiotic bacteria.
Volume 327(5969)
Pages 1122-6
Published 2010-2-26
DOI 10.1126/science.1184057
PII 327/5969/1122
PMID 20185722
MeSH Amino Acid Sequence Anti-Bacterial Agents / pharmacology Cell Division Cell Membrane / metabolism Cytosol / metabolism Genes, Plant Loteae / genetics Loteae / metabolism Loteae / microbiology Medicago truncatula / genetics Medicago truncatula / metabolism* Medicago truncatula / microbiology* Molecular Sequence Data Nitrogen Fixation Peptides / chemistry Peptides / genetics Peptides / metabolism* Peptides / pharmacology Plant Proteins / chemistry Plant Proteins / genetics Plant Proteins / metabolism* Plants, Genetically Modified Protein Transport Root Nodules, Plant / metabolism Root Nodules, Plant / microbiology Sinorhizobium meliloti / cytology* Sinorhizobium meliloti / drug effects Sinorhizobium meliloti / physiology* Symbiosis*
IF 41.058
Times Cited 202
WOS Category PLANT SCIENCES
Resource
Lotus / Glycine Miyakojima MG-20