RRC ID 30963
Author Takos AM, Knudsen C, Lai D, Kannangara R, Mikkelsen L, Motawia MS, Olsen CE, Sato S, Tabata S, Jørgensen K, Møller BL, Rook F.
Title Genomic clustering of cyanogenic glucoside biosynthetic genes aids their identification in Lotus japonicus and suggests the repeated evolution of this chemical defence pathway.
Journal Plant J.
Abstract Cyanogenic glucosides are amino acid-derived defence compounds found in a large number of vascular plants. Their hydrolysis by specific β-glucosidases following tissue damage results in the release of hydrogen cyanide. The cyanogenesis deficient1 (cyd1) mutant of Lotus japonicus carries a partial deletion of the CYP79D3 gene, which encodes a cytochrome P450 enzyme that is responsible for the first step in cyanogenic glucoside biosynthesis. The genomic region surrounding CYP79D3 contains genes encoding the CYP736A2 protein and the UDP-glycosyltransferase UGT85K3. In combination with CYP79D3, these genes encode the enzymes that constitute the entire pathway for cyanogenic glucoside biosynthesis. The biosynthetic genes for cyanogenic glucoside biosynthesis are also co-localized in cassava (Manihot esculenta) and sorghum (Sorghum bicolor), but the three gene clusters show no other similarities. Although the individual enzymes encoded by the biosynthetic genes in these three plant species are related, they are not necessarily orthologous. The independent evolution of cyanogenic glucoside biosynthesis in several higher plant lineages by the repeated recruitment of members from similar gene families, such as the CYP79s, is a likely scenario.
Volume 68(2)
Pages 273-86
Published 2011-10
DOI 10.1111/j.1365-313X.2011.04685.x
PMID 21707799
MeSH Biological Evolution Cytochrome P-450 Enzyme System / genetics Cytochrome P-450 Enzyme System / metabolism DNA, Complementary / genetics Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Genome, Plant / genetics Glucosides / biosynthesis* Glucosides / chemistry Glucosides / genetics Glucosides / metabolism Glucosyltransferases / genetics Glucosyltransferases / metabolism Glycosides / chemistry Glycosides / metabolism Hydrogen Cyanide / metabolism Loteae / enzymology Loteae / genetics* Loteae / metabolism Manihot / enzymology Manihot / genetics* Manihot / metabolism Molecular Structure Multigene Family* Mutation Nitriles / chemistry Nitriles / metabolism Phylogeny Plant Leaves / enzymology Plant Leaves / genetics Plant Leaves / metabolism Plant Proteins / genetics* Plant Proteins / metabolism RNA, Plant / genetics Sorghum / enzymology Sorghum / genetics* Sorghum / metabolism Tobacco / genetics Tobacco / metabolism
IF 5.775
Times Cited 58
Lotus / Glycine L. japonicus Miyakojima MG-20