RRC ID 30973
Author Hakoyama T, Oi R, Hazuma K, Suga E, Adachi Y, Kobayashi M, Akai R, Sato S, Fukai E, Tabata S, Shibata S, Wu GJ, Hase Y, Tanaka A, Kawaguchi M, Kouchi H, Umehara Y, Suganuma N.
Title The SNARE protein SYP71 expressed in vascular tissues is involved in symbiotic nitrogen fixation in Lotus japonicus nodules.
Journal Plant Physiol.
Abstract Soluble N-Ethylmaleimide Sensitive Factor Attachment Protein Receptor (SNARE) proteins are crucial for signal transduction and development in plants. Here, we investigate a Lotus japonicus symbiotic mutant defective in one of the SNARE proteins. When in symbiosis with rhizobia, the growth of the mutant was retarded compared with that of the wild-type plant. Although the mutant formed nodules, these exhibited lower nitrogen fixation activity than the wild type. The rhizobia were able to invade nodule cells, but enlarged symbiosomes were observed in the infected cells. The causal gene, designated LjSYP71 (for L. japonicus syntaxin of plants71), was identified by map-based cloning and shown to encode a Qc-SNARE protein homologous to Arabidopsis (Arabidopsis thaliana) SYP71. LjSYP71 was expressed ubiquitously in shoot, roots, and nodules, and transcripts were detected in the vascular tissues. In the mutant, no other visible defects in plant morphology were observed. Furthermore, in the presence of combined nitrogen, the mutant plant grew almost as well as the wild type. These results suggest that the vascular tissues expressing LjSYP71 play a pivotal role in symbiotic nitrogen fixation in L. japonicus nodules.
Volume 160(2)
Pages 897-905
Published 2012-10
DOI 10.1104/pp.112.200782
PII pp.112.200782
PMID 22858633
PMC PMC3461563
MeSH Chromosome Mapping Cloning, Molecular Crosses, Genetic Gene Expression Regulation, Plant Genes, Plant Genetic Complementation Test Loteae / genetics Loteae / metabolism* Loteae / microbiology Mesorhizobium / growth & development Microscopy, Electron, Transmission Mutagenesis Nitrogen Fixation* Phylogeny Plant Proteins / genetics Plant Proteins / metabolism Plant Shoots / genetics Plant Shoots / metabolism Plant Vascular Bundle / genetics Plant Vascular Bundle / metabolism* Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Plants, Genetically Modified / microbiology Qc-SNARE Proteins / genetics Qc-SNARE Proteins / metabolism* Root Nodules, Plant / genetics Root Nodules, Plant / metabolism Root Nodules, Plant / microbiology Symbiosis*
IF 5.949
Times Cited 14
WOS Category PLANT SCIENCES
Resource
Lotus / Glycine EST clones MR020b12 MPD004c03