RRC ID 30977
Author Takanashi K, Takahashi H, Sakurai N, Sugiyama A, Suzuki H, Shibata D, Nakazono M, Yazaki K.
Title Tissue-specific transcriptome analysis in nodules of Lotus japonicus.
Journal Mol Plant Microbe Interact
Abstract Legume plants can establish symbiotic nitrogen fixation (SNF) with rhizobia mostly in root nodules, where rhizobia-infected cells are accompanied by uninfected cells in a mosaic pattern. Inside the mature nodules of the legume, carbon and nitrogen nutrients between host plant cells and their resident bacteria are actively exchanged. To elucidate the metabolite dynamics relevant for SNF in nodules, three tissues from a nodule of a model legume, Lotus japonicus, were isolated using laser microdissesction, and transcriptome analysis was done by an oligoarray of 60-mer length representing 21,495 genes. In our tissue-specific profiling, many genes were identified as being expressed in nodules in a spatial-specific manner. Among them, genes coding for metabolic enzymes were classified according to their function, and detailed data analysis showed that a secondary metabolic pathway was highly activated in the nodule cortex. In particular, a number of metabolic genes for a phenylpropanoid pathway were found as highly expressed genes accompanied by those encoding putative transporters of secondary metabolites. These data suggest the involvement of a novel physiological function of phenylpropanoids in SNF. Moreover, five representative genes were selected, and detailed tissue-specific expression was characterized by promoter-β-glucuronidase experiments. Our results provide a new data source for investigation of both nodule differentiation and tissue-specific physiological functions in nodules.
Volume 25(7)
Pages 869-76
Published 2012-7-1
DOI 10.1094/MPMI-01-12-0011-R
PMID 22432875
MeSH Gene Expression Profiling / methods* Gene Expression Regulation, Enzymologic / genetics Gene Expression Regulation, Plant / genetics* Genes, Plant / genetics* Glucuronidase / genetics Glucuronidase / metabolism Lotus / genetics* Lotus / metabolism Lotus / microbiology Models, Biological Nitrogen Fixation Oligonucleotide Array Sequence Analysis Organ Specificity / genetics RNA, Plant / genetics Rhizobium / metabolism Root Nodules, Plant / genetics* Root Nodules, Plant / metabolism Root Nodules, Plant / microbiology Symbiosis Transcriptome*
IF 3.696
Times Cited 27
Lotus / Glycine L. japonicus