論文 - 詳細
| RRC ID | 30981 |
|---|---|
| 著者 | Bunsupa S, Katayama K, Ikeura E, Oikawa A, Toyooka K, Saito K, Yamazaki M. |
| タイトル | Lysine decarboxylase catalyzes the first step of quinolizidine alkaloid biosynthesis and coevolved with alkaloid production in leguminosae. |
| ジャーナル | Plant Cell |
| Abstract |
Lysine decarboxylase (LDC) catalyzes the first-step in the biosynthetic pathway of quinolizidine alkaloids (QAs), which form a distinct, large family of plant alkaloids. A cDNA of lysine/ornithine decarboxylase (L/ODC) was isolated by differential transcript screening in QA-producing and nonproducing cultivars of Lupinus angustifolius. We also obtained L/ODC cDNAs from four other QA-producing plants, Sophora flavescens, Echinosophora koreensis, Thermopsis chinensis, and Baptisia australis. These L/ODCs form a phylogenetically distinct subclade in the family of plant ornithine decarboxylases. Recombinant L/ODCs from QA-producing plants preferentially or equally catalyzed the decarboxylation of L-lysine and L-ornithine. L. angustifolius L/ODC (La-L/ODC) was found to be localized in chloroplasts, as suggested by the transient expression of a fusion protein of La-L/ODC fused to the N terminus of green fluorescent protein in Arabidopsis thaliana. Transgenic tobacco (Nicotiana tabacum) suspension cells and hairy roots produced enhanced levels of cadaverine-derived alkaloids, and transgenic Arabidopsis plants expressing (La-L/ODC) produced enhanced levels of cadaverine, indicating the involvement of this enzyme in lysine decarboxylation to form cadaverine. Site-directed mutagenesis and protein modeling studies revealed a structural basis for preferential LDC activity, suggesting an evolutionary implication of L/ODC in the QA-producing plants. |
| 巻・号 | 24(3) |
| ページ | 1202-16 |
| 公開日 | 2012-3-1 |
| DOI | 10.1105/tpc.112.095885 |
| PII | tpc.112.095885 |
| PMID | 22415272 |
| PMC | PMC3336119 |
| MeSH | Alkaloids / biosynthesis* Arabidopsis / metabolism Cadaverine / metabolism Carboxy-Lyases / genetics Carboxy-Lyases / metabolism* Chloroplasts / enzymology Cloning, Molecular Decarboxylation Lupinus / enzymology* Lupinus / genetics Lupinus / metabolism Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Ornithine Decarboxylase / genetics Ornithine Decarboxylase / metabolism Plant Proteins / genetics Plant Proteins / metabolism Plants, Genetically Modified / enzymology Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Quinolizidines / metabolism* Substrate Specificity Tobacco / metabolism |
| IF | 9.618 |
| 引用数 | 55 |
| WOS 分野 | PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | B01151 Gifu B-129 |