RRC ID 30981
Author Bunsupa S, Katayama K, Ikeura E, Oikawa A, Toyooka K, Saito K, Yamazaki M.
Title Lysine decarboxylase catalyzes the first step of quinolizidine alkaloid biosynthesis and coevolved with alkaloid production in leguminosae.
Journal Plant Cell
Abstract Lysine decarboxylase (LDC) catalyzes the first-step in the biosynthetic pathway of quinolizidine alkaloids (QAs), which form a distinct, large family of plant alkaloids. A cDNA of lysine/ornithine decarboxylase (L/ODC) was isolated by differential transcript screening in QA-producing and nonproducing cultivars of Lupinus angustifolius. We also obtained L/ODC cDNAs from four other QA-producing plants, Sophora flavescens, Echinosophora koreensis, Thermopsis chinensis, and Baptisia australis. These L/ODCs form a phylogenetically distinct subclade in the family of plant ornithine decarboxylases. Recombinant L/ODCs from QA-producing plants preferentially or equally catalyzed the decarboxylation of L-lysine and L-ornithine. L. angustifolius L/ODC (La-L/ODC) was found to be localized in chloroplasts, as suggested by the transient expression of a fusion protein of La-L/ODC fused to the N terminus of green fluorescent protein in Arabidopsis thaliana. Transgenic tobacco (Nicotiana tabacum) suspension cells and hairy roots produced enhanced levels of cadaverine-derived alkaloids, and transgenic Arabidopsis plants expressing (La-L/ODC) produced enhanced levels of cadaverine, indicating the involvement of this enzyme in lysine decarboxylation to form cadaverine. Site-directed mutagenesis and protein modeling studies revealed a structural basis for preferential LDC activity, suggesting an evolutionary implication of L/ODC in the QA-producing plants.
Volume 24(3)
Pages 1202-16
Published 2012-3
DOI 10.1105/tpc.112.095885
PII tpc.112.095885
PMID 22415272
PMC PMC3336119
MeSH Alkaloids / biosynthesis* Arabidopsis / metabolism Cadaverine / metabolism Carboxy-Lyases / genetics Carboxy-Lyases / metabolism* Chloroplasts / enzymology Cloning, Molecular Decarboxylation Lupinus / enzymology* Lupinus / genetics Lupinus / metabolism Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Ornithine Decarboxylase / genetics Ornithine Decarboxylase / metabolism Plant Proteins / genetics Plant Proteins / metabolism Plants, Genetically Modified / enzymology Plants, Genetically Modified / genetics Plants, Genetically Modified / metabolism Quinolizidines / metabolism* Substrate Specificity Tobacco / metabolism
IF 8.228
Times Cited 25
WOS Category PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Lotus / Glycine B01151 Gifu B-129