RRC ID 30997
Author Ampomah OY, Jensen JB.
Title The trehalose utilization gene thuA ortholog in Mesorhizobium loti does not influence competitiveness for nodulation on Lotus spp.
Journal World J. Microbiol. Biotechnol.
Abstract Competitiveness for nodulation is a desirable trait in rhizobia strains used as inoculant. In Sinorhizobium meliloti 1021 mutation in either of the trehalose utilization genes thuA or thuB influences its competitiveness for root colonization and nodule occupancy depending on the interacting host. We have therefore investigated whether mutation in the thuA ortholog in Mesorhizobium loti MAFF303099 also leads to a similar competitive phenotype on its hosts. The results show that M. loti thuA mutant Ml7023 was symbiotically effective and was as competitive as the wild type in colonization and nodule occupancy on Lotus corniculatus and Lotus japonicus. The thuA gene in M. loti was not induced during root colonization or in the infection threads unlike in S. meliloti, despite its induction by trehalose and high osmolarity in in vitro assays.
Volume 30(3)
Pages 1129-34
Published 2014-3
DOI 10.1007/s11274-013-1527-6
PMID 24142427
MeSH Bacterial Proteins / genetics Bacterial Proteins / metabolism* Loteae / microbiology* Mesorhizobium / enzymology* Mesorhizobium / growth & development* Mesorhizobium / metabolism Mesorhizobium / physiology Mutation Plant Root Nodulation* Plant Roots / microbiology Symbiosis Trehalose / metabolism*
IF 2.1
Times Cited 0
Lotus / Glycine Gifu B-129