RRC ID 31000
Author Tek AL, Kashihara K, Murata M, Nagaki K.
Title Identification of the centromere-specific histone H3 variant in Lotus japonicus.
Journal Gene
Abstract The centromere is a structurally and functionally specialized region present on every eukaryotic chromosome. Lotus japonicus is a model legume species for which there is very limited information on the centromere structure. Here we cloned and characterized the L. japonicus homolog of the centromere-specific histone H3 gene (LjCenH3) encoding a 159-amino acid protein. Using an Agrobacterium-based transformation system, LjCenH3 tagged with a green fluorescent protein was transferred into L. japonicus cells. The centromeric position of LjCENH3 protein was revealed on L. japonicus metaphase chromosomes by an immunofluorescence assay. The identification of LjCenH3 as a critical centromere landmark could pave the way for a better understanding of centromere structure in this model and other agriculturally important legume species.
Volume 538(1)
Pages 8-11
Published 2014-3-15
DOI 10.1016/j.gene.2014.01.034
PII S0378-1119(14)00055-9
PMID 24462968
MeSH Amino Acid Sequence Base Sequence Centromere / chemistry Centromere / metabolism* Histones / chemistry Histones / genetics* Histones / metabolism Lotus / chemistry Lotus / genetics* Lotus / metabolism Molecular Sequence Data Plant Proteins / chemistry Plant Proteins / genetics* Plant Proteins / metabolism Protein Binding
IF 2.984
Times Cited 5
Lotus / Glycine L. japonicus