論文 - 詳細
| RRC ID | 3102 |
|---|---|
| 著者 | Alejandro S, Rodríguez PL, Bellés JM, Yenush L, García-Sanchez MJ, Fernández JA, Serrano R. |
| タイトル | An Arabidopsis quiescin-sulfhydryl oxidase regulates cation homeostasis at the root symplast-xylem interface. |
| ジャーナル | EMBO J |
| Abstract |
A genetic screen of Arabidopsis 'activation-tagging' mutant collection based on tolerance to norspermidine resulted in a dominant mutant (par1-1D) with increased expression of the QSO2 gene (At1g15020), encoding a member of the quiescin-sulfhydryl oxidase (QSO) family. The par1-1D mutant and transgenic plants overexpressing QSO2 cDNA grow better than wild-type Arabidopsis in media with toxic cations (polyamines, Li(+) and Na(+)) or reduced K(+) concentrations. This correlates with a decrease in the accumulation of toxic cations and an increase in the accumulation of K(+) in xylem sap and shoots. Conversely, three independent loss-of-function mutants of QSO2 exhibit phenotypes opposite to those of par1-1D. QSO2 is mostly expressed in roots and is upregulated by K(+) starvation. A QSO2Colon, two colonsGFP fusion ectopically expressed in leaf epidermis localized at the cell wall. The recombinant QSO2 protein, produced in yeast in secreted form, exhibits disulfhydryl oxidase activity. A plausible mechanism of QSO2 action consists on the activation of root systems loading K(+) into xylem, but different from the SKOR channel, which is not required for QSO2 action. These results uncover QSOs as novel regulators of ion homeostasis. |
| 巻・号 | 26(13) |
| ページ | 3203-15 |
| 公開日 | 2007-7-11 |
| DOI | 10.1038/sj.emboj.7601757 |
| PII | 7601757 |
| PMID | 17568770 |
| PMC | PMC1914105 |
| MeSH | Arabidopsis / drug effects Arabidopsis / genetics Arabidopsis / growth & development Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cations / chemistry Cations / metabolism Cations / toxicity DNA, Plant / genetics Gene Expression Regulation, Plant Homeostasis* / drug effects Mutation / genetics Oxidoreductases Acting on Sulfur Group Donors / genetics Oxidoreductases Acting on Sulfur Group Donors / metabolism* Plant Roots / drug effects Plant Roots / genetics Plant Roots / growth & development Plant Roots / metabolism* Plants, Genetically Modified Potassium / chemistry Potassium / metabolism Sensitivity and Specificity Spermidine / analogs & derivatives Spermidine / pharmacology Xylem / metabolism* |
| IF | 9.889 |
| 引用数 | 22 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda04266 |