RRC ID 31030
Author Gospodaryov DV, Lushchak OV, Rovenko BM, Perkhulyn NV, Gerards M, Tuomela T, Jacobs HT.
Title Ciona intestinalis NADH dehydrogenase NDX confers stress-resistance and extended lifespan on Drosophila.
Journal Biochim. Biophys. Acta
Abstract An assembled cDNA coding for the putative single-subunit NADH dehydrogenase (NDX) of Ciona intestinalis was introduced into Drosophila melanogaster. The encoded protein was found to localize to mitochondria and to confer rotenone-insensitive substrate oxidation in organello. Transgenic flies exhibited increased resistance to menadione, starvation and temperature stress, and manifested a sex and diet-dependent increase in mean lifespan of 20-50%. However, NDX was able only weakly to complement the phenotypes produced by the knockdown of complex I subunits.
Volume 1837(11)
Pages 1861-1869
Published 2014-11
DOI 10.1016/j.bbabio.2014.08.001
PII S0005-2728(14)00561-1
PMID 25124484
IF 3.438
Times Cited 3
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
DNA material
C.intestinalis / (O.japonicus) Ciona intestinalis EST Clones cibd016c12 ciad062k07 ciem809l11