| RRC ID |
3109
|
| Author |
Nakamura Y, Kimura A, Saga H, Oikawa A, Shinbo Y, Kai K, Sakurai N, Suzuki H, Kitayama M, Shibata D, Kanaya S, Ohta D.
|
| Title |
Differential metabolomics unraveling light/dark regulation of metabolic activities in Arabidopsis cell culture.
|
| Journal |
Planta
|
| Abstract |
Differential metabolomics based on a non-targeted FT-ICR/MS analysis demonstrated metabolite accumulation patterns reflecting light/dark conditions in Arabidopsis T87 cell culture. First, FT-ICR/MS data sets were converted into metabolome information using the Dr.DMASS software (http://kanaya.naist.jp/DrDMASS/). A quick search of a metabolite-species database, KNApSAcK (http://kanaya.naist.jp/KNApSAcK/), was implemented to assign metabolite candidates to each accurate MS data (<1 ppm) through the prediction of molecular formulas, and the candidate structures were further studied using MS/MS analyses. Specific metabolites representing the culture conditions included sugars, phenylpropanoid derivatives, flavonol aglycons, and a plastid nonmevalonate pathway intermediate. Transcriptomics data were obtained in parallel and analyzed using a transcriptome analysis tool, KaPPA-View (http://kpv.kazusa.or.jp/kappa-view/). The specific accumulation patterns of flavonol aglycons were in good agreement with the light/dark regulation of a cytochrome P450 gene, CYP75B, and the build-up of 2-C-methyl-D-erythritol 4-phosphate, a nonmevalonate pathway intermediate, in the light grown cells was also consistent with a gene expression profile. The differential metablomics scheme based on the FT-ICR/MS metabolomics can serve as an evaluation system of metabolic activities contributing to successful identification and proper manipulation of key enzymatic steps in metabolic engineering studies.
|
| Volume |
227(1)
|
| Pages |
57-66
|
| Published |
2007-12-1
|
| DOI |
10.1007/s00425-007-0594-z
|
| PMID |
17701204
|
| MeSH |
Arabidopsis / cytology
Arabidopsis / genetics*
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism*
Cells, Cultured
Gene Expression Regulation, Plant / radiation effects
Light
Mass Spectrometry
Models, Biological
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Tandem Mass Spectrometry
|
| IF |
3.39
|
| Times Cited |
36
|
|
WOS Category
|
PLANT SCIENCES
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
rpc00008 |
