論文 - 詳細
| RRC ID | 31333 |
|---|---|
| 著者 | Su Y, Wang J, Shi M, Niu X, Yu X, Gao L, Zhang X, Chen L, Zhang W. |
| タイトル | Metabolomic and network analysis of astaxanthin-producing Haematococcus pluvialis under various stress conditions. |
| ジャーナル | Bioresour Technol |
| Abstract |
Various combinations of acetate (Ac), Fe(2+) and high light (HL) stress conditions were evaluated to maximize astaxanthin accumulation and biomass production in Haematococcus pluvialis, and then GC-MS and LC-MS based metabolomics were applied to determine molecular mechanisms responsible for enhancing astaxanthin accumulation under the stress conditions. With the optimized analytical protocols, the GC-MS and LC-MS analyses allowed identification of 93 stable and 24 unstable intracellular metabolites from H. pluvialis, respectively. In addition, a metabolic network was constructed based on GC-MS metabolomic datasets using a weighted correlation network analysis (WGCNA) approach. The network analysis uncovered 2, 1 and 1 distinguished metabolic modules highly associated with HL, Fe(2+) & HL, and Ac & Fe(2+) & HL conditions, respectively. Finally, LC-MS analysis found that AKG, Glu and R5P may be metabolites associated with the Fe(2+) & HL condition. The study provided the first metabolomic view of cell growth and astaxanthin accumulation in H. pluvialis. |
| 巻・号 | 170 |
| ページ | 522-529 |
| 公開日 | 2014-10-1 |
| DOI | 10.1016/j.biortech.2014.08.018 |
| PII | S0960-8524(14)01125-0 |
| PMID | 25164345 |
| MeSH | Chlorophyta / metabolism Chlorophyta / physiology* Chromatography, Liquid / methods Gas Chromatography-Mass Spectrometry / methods Metabolic Networks and Pathways / genetics Metabolic Networks and Pathways / physiology* Metabolome / genetics Metabolome / physiology* Metabolomics / methods* Stress, Physiological / physiology* Xanthophylls / biosynthesis Xanthophylls / genetics Xanthophylls / metabolism |
| IF | 7.539 |
| 引用数 | 46 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY AGRICULTURAL ENGINEERING ENERGY & FUELS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-144 |