RRC ID 31333
Author Su Y, Wang J, Shi M, Niu X, Yu X, Gao L, Zhang X, Chen L, Zhang W.
Title Metabolomic and network analysis of astaxanthin-producing Haematococcus pluvialis under various stress conditions.
Journal Bioresour Technol
Abstract Various combinations of acetate (Ac), Fe(2+) and high light (HL) stress conditions were evaluated to maximize astaxanthin accumulation and biomass production in Haematococcus pluvialis, and then GC-MS and LC-MS based metabolomics were applied to determine molecular mechanisms responsible for enhancing astaxanthin accumulation under the stress conditions. With the optimized analytical protocols, the GC-MS and LC-MS analyses allowed identification of 93 stable and 24 unstable intracellular metabolites from H. pluvialis, respectively. In addition, a metabolic network was constructed based on GC-MS metabolomic datasets using a weighted correlation network analysis (WGCNA) approach. The network analysis uncovered 2, 1 and 1 distinguished metabolic modules highly associated with HL, Fe(2+) & HL, and Ac & Fe(2+) & HL conditions, respectively. Finally, LC-MS analysis found that AKG, Glu and R5P may be metabolites associated with the Fe(2+) & HL condition. The study provided the first metabolomic view of cell growth and astaxanthin accumulation in H. pluvialis.
Volume 170
Pages 522-529
Published 2014-10-1
DOI 10.1016/j.biortech.2014.08.018
PII S0960-8524(14)01125-0
PMID 25164345
MeSH Chlorophyta / metabolism Chlorophyta / physiology* Chromatography, Liquid / methods Gas Chromatography-Mass Spectrometry / methods Metabolic Networks and Pathways / genetics Metabolic Networks and Pathways / physiology* Metabolome / genetics Metabolome / physiology* Metabolomics / methods* Stress, Physiological / physiology* Xanthophylls / biosynthesis Xanthophylls / genetics Xanthophylls / metabolism
IF 7.539
Times Cited 46
Algae NIES-144