論文 - 詳細
| RRC ID | 31334 |
|---|---|
| 著者 | Satou Y, Hirayama K, Mita K, Fujie M, Chiba S, Yoshida R, Endo T, Sasakura Y, Inaba K, Satoh N. |
| タイトル | Sustained heterozygosity across a self-incompatibility locus in an inbred ascidian. |
| ジャーナル | Mol Biol Evol |
| Abstract |
Because self-incompatibility loci are maintained heterozygous and recombination within self-incompatibility loci would be disadvantageous, self-incompatibility loci are thought to contribute to structural and functional differentiation of chromosomes. Although the hermaphrodite chordate, Ciona intestinalis, has two self-incompatibility genes, this incompatibility system is incomplete and self-fertilization occurs under laboratory conditions. Here, we established an inbred strain of C. intestinalis by repeated self-fertilization. Decoding genome sequences of sibling animals of this strain identified a 2.4-Mbheterozygous region on chromosome 7. A self-incompatibility gene, Themis-B, was encoded within this region. This observation implied that this self-incompatibility locus and the linkage disequilibrium of its flanking region contribute to the formation of the 2.4-Mb heterozygous region, probably through recombination suppression. We showed that different individuals in natural populations had different numbers and different combinations of Themis-B variants, and that the rate of self-fertilization varied among these animals. Our result explains why self-fertilization occurs under laboratory conditions. It also supports the concept that the Themis-B locus is preferentially retained heterozygous in the inbred line and contributes to the formation of the 2.4-Mb heterozygous region. High structural variations might suppress recombination, and this long heterozygous region might represent a preliminary stage of structural differentiation of chromosomes. |
| 巻・号 | 32(1) |
| ページ | 81-90 |
| 公開日 | 2015-1-1 |
| DOI | 10.1093/molbev/msu268 |
| PII | msu268 |
| PMID | 25234703 |
| MeSH | Animals Animals, Inbred Strains / genetics* Animals, Inbred Strains / physiology Chromosomes Ciona intestinalis / genetics* Ciona intestinalis / physiology Genetic Loci Genetic Variation Heterozygote* Self-Fertilization Sequence Analysis, DNA |
| IF | 11.062 |
| 引用数 | 3 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY EVOLUTIONARY BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |