RRC ID 31352
著者 Ishikawa T, Taniguchi Y, Okada T, Takeda S, Mori K.
タイトル Vertebrate unfolded protein response: mammalian signaling pathways are conserved in Medaka fish.
ジャーナル Cell Struct Funct
Abstract The accumulation of unfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR). The ER stress signal is sensed and transmitted by a transmembrane protein(s) in the ER. The number of these transducers has increased with evolution, one in yeast, three in worm and fly, and five in mammals. Here, we examined medaka fish, Oryzias latipes, as a vertebrate model organism, and found that the medaka genome encodes five UPR transducers. Analysis of a medaka embryonic cell line revealed that the mammalian UPR signaling mechanisms are very well conserved. Thus, XBP1 mRNA, which encodes the transcription factor XBP1 downstream of the IRE1 pathway, was spliced in response to ER stress, resulting in production of the active form of XBP1. Translation was generally attenuated in response to ER stress, which paradoxically induced the translation of ATF4, the transcription factor downstream of the PERK pathway. ATF6 was constitutively synthesized as a transmembrane protein and activated by ER stress-induced proteolysis. Results obtained with the overexpression of active ATF6α, ATF6β, and XBP1 strongly suggested that ATF6α plays a major role in upregulating the major ER chaperone BiP, contrary to the case in non-vertebrates, in which the IRE1 pathway is essential to the induction of BiP. Physiological ER stress occurring during embryonic development was visualized using transgenic medaka carrying the enhanced green fluorescent protein gene under the control of the BiP promoter. Thus, analysis of the vertebrate UPR using medaka will help provide a more comprehensive understanding of the biology and physiology of the UPR.
巻・号 36(2)
ページ 247-59
公開日 2011-1-1
DOI 10.1247/csf.11036
PII JST.JSTAGE/csf/11036
PMID 22067999
MeSH Animals Endoplasmic Reticulum / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Models, Animal Oryzias / metabolism* Protein Unfolding RNA, Messenger / genetics RNA, Messenger / metabolism Signal Transduction* Unfolded Protein Response / physiology*
IF 2.429
引用数 27
WOS 分野 CELL BIOLOGY
リソース情報
メダカ Tg(PBiP-EGFP) (TG998) Tg(PBiP-EGFP)2 (TG999) Tg(PBiP-EGFP)3 (TG1000) Tg(PBiP-EGFP)4 (TG1001) Tg(PBiP-EGFP)5 (TG1002) Tg(PIRE1alpha-EGFP) (TG1006) Tg(PIRE1alpha-EGFP)2 (TG1007)