RRC ID 31357
Author Mise-Omata S, Obata Y, Doi TS.
Title p100, a precursor of NF-κB2, inhibits c-Rel and reduces the expression of IL-23 in dendritic cells.
Journal Biochem. Biophys. Res. Commun.
Abstract Nuclear factor κB regulates various genes involved in the immune response, inflammation, cell survival, and development. NF-κB activation is controlled by proteins possessing ankyrin repeats, such as IκBs. A precursor of the NF-κB2 (p52) subunit, p100, contains ankyrin repeats in its C-terminal portion and has been found to act as a cytoplasmic inhibitor of RelA in the canonical pathway of NF-κB activation. Here, we demonstrate that p100 also suppresses c-Rel function in dendritic cells. Expression of the p19 and p40 subunits of IL-23, a c-Rel-dependent cytokine, was enhanced in p100-deficient cells, although expression of a RelA-dependent cytokine, TNF-α, was reduced. Nuclear translocation of c-Rel was enhanced in p100-deficient cells. p100, and not the processed p52 form, associated with c-Rel in the steady state and dissociated immediately after lipopolysaccharide stimulation in wild-type dendritic cells. Four hours after the stimulation, p100 was newly synthesized and associated with c-Rel again. In cells expressing both c-Rel and RelA, c-Rel is preferentially suppressed by p100.
Volume 453(3)
Pages 332-7
Published 2014-10-24
DOI 10.1016/j.bbrc.2014.09.143
PII S0006-291X(14)01786-0
PMID 25305492
MeSH Animals Base Sequence DNA Primers Dendritic Cells / metabolism* HEK293 Cells Humans Interleukin-23 / metabolism* Mice NF-kappa B p52 Subunit / genetics NF-kappa B p52 Subunit / physiology* Polymerase Chain Reaction Proto-Oncogene Proteins c-rel / antagonists & inhibitors*
IF 2.559
Times Cited 3
DNA material p19-937-Luc (RDB06302)