論文 - 詳細
| RRC ID | 31357 |
|---|---|
| 著者 | Mise-Omata S, Obata Y, Doi TS. |
| タイトル | p100, a precursor of NF-κB2, inhibits c-Rel and reduces the expression of IL-23 in dendritic cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Nuclear factor κB regulates various genes involved in the immune response, inflammation, cell survival, and development. NF-κB activation is controlled by proteins possessing ankyrin repeats, such as IκBs. A precursor of the NF-κB2 (p52) subunit, p100, contains ankyrin repeats in its C-terminal portion and has been found to act as a cytoplasmic inhibitor of RelA in the canonical pathway of NF-κB activation. Here, we demonstrate that p100 also suppresses c-Rel function in dendritic cells. Expression of the p19 and p40 subunits of IL-23, a c-Rel-dependent cytokine, was enhanced in p100-deficient cells, although expression of a RelA-dependent cytokine, TNF-α, was reduced. Nuclear translocation of c-Rel was enhanced in p100-deficient cells. p100, and not the processed p52 form, associated with c-Rel in the steady state and dissociated immediately after lipopolysaccharide stimulation in wild-type dendritic cells. Four hours after the stimulation, p100 was newly synthesized and associated with c-Rel again. In cells expressing both c-Rel and RelA, c-Rel is preferentially suppressed by p100. |
| 巻・号 | 453(3) |
| ページ | 332-7 |
| 公開日 | 2014-10-24 |
| DOI | 10.1016/j.bbrc.2014.09.143 |
| PII | S0006-291X(14)01786-0 |
| PMID | 25305492 |
| MeSH | Animals Base Sequence DNA Primers Dendritic Cells / metabolism* HEK293 Cells Humans Interleukin-23 / metabolism* Mice NF-kappa B p52 Subunit / genetics NF-kappa B p52 Subunit / physiology* Polymerase Chain Reaction Proto-Oncogene Proteins c-rel / antagonists & inhibitors* |
| IF | 2.985 |
| 引用数 | 7 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | p19-937-Luc (RDB06302) |