RRC ID 31358
Author Hayashi K, Ogiyama Y, Yokomi K, Nakagawa T, Kaino T, Kawamukai M.
Title Functional conservation of coenzyme Q biosynthetic genes among yeasts, plants, and humans.
Journal PLoS ONE
Abstract Coenzyme Q (CoQ) is an essential factor for aerobic growth and oxidative phosphorylation in the electron transport system. The biosynthetic pathway for CoQ has been proposed mainly from biochemical and genetic analyses of Escherichia coli and Saccharomyces cerevisiae; however, the biosynthetic pathway in higher eukaryotes has been explored in only a limited number of studies. We previously reported the roles of several genes involved in CoQ synthesis in the fission yeast Schizosaccharomyces pombe. Here, we expand these findings by identifying ten genes (dps1, dlp1, ppt1, and coq3-9) that are required for CoQ synthesis. CoQ10-deficient S. pombe coq deletion strains were generated and characterized. All mutant fission yeast strains were sensitive to oxidative stress, produced a large amount of sulfide, required an antioxidant to grow on minimal medium, and did not survive at the stationary phase. To compare the biosynthetic pathway of CoQ in fission yeast with that in higher eukaryotes, the ability of CoQ biosynthetic genes from humans and plants (Arabidopsis thaliana) to functionally complement the S. pombe coq deletion strains was determined. With the exception of COQ9, expression of all other human and plant COQ genes recovered CoQ10 production by the fission yeast coq deletion strains, although the addition of a mitochondrial targeting sequence was required for human COQ3 and COQ7, as well as A. thaliana COQ6. In summary, this study describes the functional conservation of CoQ biosynthetic genes between yeasts, humans, and plants.
Volume 9(6)
Pages e99038
Published 2014
DOI 10.1371/journal.pone.0099038
PII PONE-D-14-08532
PMID 24911838
PMC PMC4049637
MeSH Arabidopsis / genetics* Arabidopsis / metabolism Biosynthetic Pathways Gene Deletion Genes, Fungal Genes, Plant Humans Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Ubiquinone / genetics* Ubiquinone / metabolism
IF 2.766
Times Cited 11
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda00862 pda05612 pda13728 pdx56847 pdz55924