RRC ID 31382
Author Nakayama S, Kaiser K, Aigaki T.
Title Ectopic expression of sex-peptide in a variety of tissues in Drosophila females using the P[GAL4] enhancer-trap system.
Journal Mol. Gen. Genet.
Abstract Sex-peptide (SP), which is secreted by the accessory gland of Drosophila males, is transferred to the female during copulation, thereby reducing her sexual appetite (receptivity to males) and stimulating ovulation/oviposition. SP is known to be taken up into the hemolymph of mated females, but it is not clear whether there are two separate target tissues, for behavioral changes and ovulation or only one target for both responses. We have employed the GAL4-UAS system to express SP transgene constructs, both in different tissues and in different cellular components of virgin females. A cytoplasmic form of SP lacking a signal sequence did not evoke any responses, even when expressed ubiquitously. In contrast, a membrane-bound form of SP induced typical post-mating behavior, indicating that SP must be outside the cell in order to exert its biological effects. A total of 204 randomly selected P[GAL4] enhancer-trap lines were screened for their ability to induce SP responses in combination with the membrane-bound SP expressed under GAL4 control. Thirty-three lines were associated with both behavioral change and stimulated ovulation. No line was associated with only one of the two responses, implying that the SP target(s) mediating the two responses are either identical, very closely located, or present in two distinct tissues with a common set of genetic determinants. Western blot analysis of head, thorax, and abdominal extracts revealed that the biological activity was correlated with expression in the head fraction.
Volume 254(4)
Pages 449-55
Published 1997-4-28
PMID 9180699
MeSH Animals Animals, Genetically Modified Blotting, Western Cloning, Molecular Drosophila Proteins* Drosophila melanogaster / chemistry Drosophila melanogaster / genetics* Drosophila melanogaster / metabolism Enhancer Elements, Genetic / genetics* Female Gene Expression Regulation* Insect Hormones / genetics* Insect Hormones / physiology Male Mutation Ovulation Peptides / genetics* Peptides / physiology Promoter Regions, Genetic / genetics Recombinant Fusion Proteins / genetics Sexual Behavior, Animal Transformation, Genetic beta-Galactosidase / genetics beta-Galactosidase / metabolism
Times Cited 41